{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:27:32Z","timestamp":1790022452837,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,7,20]],"date-time":"2022-07-20T00:00:00Z","timestamp":1658275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["92051104"],"award-info":[{"award-number":["92051104"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Alkane constitutes major fractions of crude oils, and its microbial aerobic degradation dominantly follows the terminal oxidation and the sub\u2010terminal pathways. However, the latter one received much less attention, especially since the related genes were yet to be fully defined. Here, we isolated a bacterium designated\n                    <jats:italic>Acinetobacter<\/jats:italic>\n                    sp. strain NyZ410, capable of growing on alkanes with a range of chain lengths and derived sub\u2010terminal oxidation products. From its genome, a\n                    <jats:styled-content>s<\/jats:styled-content>\n                    econdary\n                    <jats:styled-content>a<\/jats:styled-content>\n                    lcohol\n                    <jats:styled-content>d<\/jats:styled-content>\n                    egradation gene cluster (\n                    <jats:italic>sad<\/jats:italic>\n                    ) was identified to be likely involved in converting the aliphatic secondary alcohols (the sub\u2010terminal oxidation products of alkanes) to the corresponding primary alcohols by removing two\u2010carbon unit. On this cluster,\n                    <jats:italic>sadC<\/jats:italic>\n                    encoded an alcohol dehydrogenase converting the aliphatic secondary alcohols to the corresponding ketones;\n                    <jats:italic>sadD<\/jats:italic>\n                    encoded a Baeyer\u2013Villiger monooxygenase catalysing the conversion of the aliphatic ketones to the corresponding esters; SadA and SadB are two esterases hydrolyzing aliphatic esters to the primary alcohols and acetic acids. Bioinformatics analyses indicated that the\n                    <jats:italic>sad<\/jats:italic>\n                    cluster was widely distributed in the genomes of probable alkane degraders, apparently coexisting (64%) with the signature enzymes AlkM and AlmA for alkane terminal oxidation in 350 bacterial genomes. It suggests that the alkane sub\u2010terminal oxidation may be more ubiquitous than previously thought.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.16124","type":"journal-article","created":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T04:19:51Z","timestamp":1657858791000},"page":"6307-6319","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Wide distribution of the\n                    <i>sad<\/i>\n                    gene cluster for sub\u2010terminal oxidation in alkane utilizers"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6905-0909","authenticated-orcid":false,"given":"Chao\u2010Fan","family":"Yin","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic &amp; Developmental Sciences, and School of Life Sciences &amp; Biotechnology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5936-0818","authenticated-orcid":false,"given":"Ying","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic &amp; Developmental Sciences, and School of Life Sciences &amp; Biotechnology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8255-7798","authenticated-orcid":false,"given":"Tao","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic &amp; Developmental Sciences, and School of Life Sciences &amp; Biotechnology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0917-5750","authenticated-orcid":false,"given":"Ning\u2010Yi","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic &amp; Developmental Sciences, and School of Life Sciences &amp; Biotechnology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1mt00048a"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msz189"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9081695"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-013-5364-1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/2021.06.20.449169"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0609650104"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr367"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.102.1.281-282.1970"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.9b03396"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00058"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13762-018-2087-y"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.top102277"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-007-0902-3"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-006-0556-6"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz239"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.11.069"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.93.4.1289-1293.1967"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst025"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21256"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep04968"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.255075"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.4.1175-1179.1998"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.01948.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-77587-4_59"},{"key":"e_1_2_8_27_1","volume-title":"Molecular cloning: a laboratory manual (2nd ed.)","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_8_28_1","unstructured":"Schrodinger LLC. 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