{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:45:59Z","timestamp":1791333959954,"version":"4.3.1"},"reference-count":56,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T00:00:00Z","timestamp":1660176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010034","name":"Walailak University","doi-asserted-by":"publisher","award":["WU\u2010IRG\u201063\u2010080"],"award-info":[{"award-number":["WU\u2010IRG\u201063\u2010080"]}],"id":[{"id":"10.13039\/501100010034","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study explored the prevalence, genetic diversity, and population structure of azole\u2010resistant\n                    <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    (AR\n                    <jats:italic>Af<\/jats:italic>\n                    ) at Walailak University in Southern Thailand. Three hundred samples were collected from dwellings and workplaces, screened for azole resistance, and tested for drug susceptibility. Molecular detection of alterations in the\n                    <jats:italic>cyp51A<\/jats:italic>\n                    gene and\n                    <jats:italic>CSP1<\/jats:italic>\n                    typing was performed. Nucleotide polymorphism and haplotype diversity were calculated, and selective neutrality tests were performed. In total, 62\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    isolates were identified, with 17 isolates displaying resistance to medical azoles. The prevalence of AR\n                    <jats:italic>Af<\/jats:italic>\n                    in the\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    isolates was 27.4%. Almost all azole\u2010resistant isolates harboured an amino acid substitution in the hotspot region of the\n                    <jats:italic>cyp51A<\/jats:italic>\n                    gene, especially at or nearby the G54 residue that has been reported as a cause of azole resistance arising from long\u2010term azole treatment. Moreover, some of the AR\n                    <jats:italic>Af<\/jats:italic>\n                    isolates harboured tandem repeats in the promoter region, which have been reported as a cause of resistance arising from the use of azole fungicides in crop protection. Finally, selective neutrality testing also suggested an impact of natural selection on DNA diversity. Therefore, we hypothesize that the factors causing the high prevalence of AR\n                    <jats:italic>Af<\/jats:italic>\n                    in this area are both in vivo\u2010 and ex vivo\u2010acquired resistance.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.16154","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T03:35:05Z","timestamp":1659497705000},"page":"4641-4651","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["High prevalence and genetic diversity of a single ancestral origin azole\u2010resistant\n                    <scp>\n                      <i>Aspergillus fumigatus<\/i>\n                    <\/scp>\n                    in indoor environments at Walailak University, Southern Thailand"],"prefix":"10.1111","volume":"24","author":[{"given":"Monlaya","family":"Daloh","sequence":"first","affiliation":[{"name":"Kapho Hospital  Kapho District Pattani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sueptrakool","family":"Wisessombat","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences Walailak University  Thasala District, Nakhonsithammarat Thailand"},{"name":"Center of Excellence Research for Melioidosis and Microorganisms (CERMM) Walailak University  Thasala District, Nakhonsithammarat Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadthanan","family":"Pinchai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Siriraj Hospital Mahidol University  Bangkoknoi, Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirijan","family":"Santajit","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences Walailak University  Thasala District, Nakhonsithammarat Thailand"},{"name":"Research Center in Tropical Pathobiology Walailak University  Thasala District, Nakhonsithammarat Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phuangthip","family":"Bhoopong","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences Walailak University  Thasala District, Nakhonsithammarat Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Areeya","family":"Soaart","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences Walailak University  Thasala District, Nakhonsithammarat Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuntida","family":"Chueajeen","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences Walailak University  Thasala District, Nakhonsithammarat Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anucha","family":"Jitlang","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences Walailak University  Thasala District, Nakhonsithammarat Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7644-5311","authenticated-orcid":false,"given":"Imran","family":"Sama\u2010ae","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences Walailak University  Thasala District, Nakhonsithammarat Thailand"},{"name":"Center of Excellence Research for Melioidosis and Microorganisms (CERMM) Walailak University  Thasala District, Nakhonsithammarat Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2014.05.009"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(20)30237-X"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep45631"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof6020091"},{"key":"e_1_2_10_6_1","unstructured":"Arendrup M. 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