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The AMO belongs to the copper\u2010containing membrane monooxygenase (CuMMO) enzyme superfamily, which also contains particulate methane monooxygenase (pMMO). Enzymes in the CuMMO superfamily are promiscuous, which results in co\u2010oxidation of alternative substrates. The phylogenetic and structural similarity between the pMMO and the archaeal AMO is well\u2010established, but there is surprisingly little information on the influence of methane and methanol on the archaeal AMO and terrestrial nitrification. The aim of this study was to examine the effects of methane and methanol on the soil ammonia\u2010oxidizing archaeon \u2018\n                    <jats:italic>Candidatus<\/jats:italic>\n                    Nitrosocosmicus franklandus C13\u2019. We demonstrate that both methane and methanol are competitive inhibitors of the archaeal AMO. The inhibition constants (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>i<\/jats:sub>\n                    ) for methane and methanol were 2.2 and 20\u2009\u03bcM, respectively, concentrations which are environmentally relevant and orders of magnitude lower than those previously reported for ammonia\u2010oxidizing bacteria. Furthermore, we demonstrate that a specific suite of proteins is upregulated and downregulated in \u2018\n                    <jats:italic>Ca.<\/jats:italic>\n                    Nitrosocosmicus franklandus C13\u2019 in the presence of methane or methanol, which provides a foundation for future studies into metabolism of one\u2010carbon (C1) compounds in ammonia\u2010oxidizing archaea.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.16316","type":"journal-article","created":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T10:55:05Z","timestamp":1672829705000},"page":"948-961","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The effect of methane and methanol on the terrestrial ammonia\u2010oxidizing archaeon \u2018\n                    <i>Candidatus<\/i>\n                    Nitrosocosmicus franklandus\n                    <scp>C13<\/scp>\n                    \u2019"],"prefix":"10.1111","volume":"25","author":[{"given":"Barbora","family":"Oudova\u2010Rivera","sequence":"first","affiliation":[{"name":"School of Biological Sciences University of East Anglia  Norwich UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chloe L.","family":"Wright","sequence":"additional","affiliation":[{"name":"School of Biological Sciences University of East Anglia  Norwich UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8577-2602","authenticated-orcid":false,"given":"Andrew T.","family":"Crombie","sequence":"additional","affiliation":[{"name":"School of Biological Sciences University of East Anglia  Norwich UK"},{"name":"School of Environmental Sciences University of East Anglia  Norwich UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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