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The LAB,\n                    <jats:italic>Lacticaseibacillus paracasei<\/jats:italic>\n                    F9\u201002 showed strong antimicrobial activity against clinical pathogens. Their proteinaceous nature was confirmed as the activity was completely abolished when treated with proteinaceous enzymes and retained during neutral pH and catalase treatment. The purified bacteriocin showed antimicrobial activity at the minimum inhibitory concentration (MIC) value of 7.56\u2009\u03bcg\/ml against vancomycin\u2010resistant\n                    <jats:italic>Enterococcus<\/jats:italic>\n                    sp. [vancomycin\u2010resistant enterococcal (VRE)], and methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA), 15.13\u2009\u03bcg\/ml against\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    ,\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    subsp\n                    <jats:italic>. enterica<\/jats:italic>\n                    serotype typhi and 30.25\u2009\u03bcg\/ml against\n                    <jats:italic>Shigella flexneri<\/jats:italic>\n                    . Present study also proved the bactericidal, non\u2010cytotoxic and non\u2010hemolytic nature of bacteriocin. Additionally, bacteriocin retained their stability under various physico\u2010chemical conditions, broad range of pH (2\u201310), temperature (40\u2013121\u00b0C), enzymes (amylase, lipase and lysozyme), surfactants [Tween\u201020, 80, 100 and sodium dodecyl sulfate (SDS)], metal ions (CaCl\n                    <jats:sub>2<\/jats:sub>\n                    , FeSO\n                    <jats:sub>4<\/jats:sub>\n                    , ZnSO\n                    <jats:sub>4<\/jats:sub>\n                    , MgSO\n                    <jats:sub>4<\/jats:sub>\n                    , MnSO\n                    <jats:sub>4<\/jats:sub>\n                    , CuCl\n                    <jats:sub>2<\/jats:sub>\n                    ) and NaCl (2%\u20138%). The molecular weight of bacteriocin (~28\u2009kDa) was determined by sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis (SDS\u2010PAGE), functional and active groups were assessed by Fourier Transform\u2010Infrared (FT\u2010IR). To our knowledge, this is the first study reporting\n                    <jats:italic>L. paracasei<\/jats:italic>\n                    from breast\u2010fed infants' faeces and assessing their antimicrobial compound, bacteriocin. The study results furnish the essential features to confirm the therapeutic potential of\n                    <jats:italic>L. paracasei<\/jats:italic>\n                    F9\u201002 bacteriocin.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.16327","type":"journal-article","created":{"date-parts":[[2022,12,24]],"date-time":"2022-12-24T02:05:00Z","timestamp":1671847500000},"page":"2882-2896","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Bactericidal and non\u2010cytotoxic activity of bacteriocin produced by\n                    <i>Lacticaseibacillus paracasei<\/i>\n                    <scp>F9<\/scp>\n                    \u201002 and evaluation of its tolerance to various physico\u2010chemical conditions"],"prefix":"10.1111","volume":"25","author":[{"given":"Senbagam","family":"Duraisamy","sequence":"first","affiliation":[{"name":"Microbial Biotechnology Laboratory, Department of Marine Biotechnology Bharathidasan University  Tiruchirappalli Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aswathy","family":"Sathyan","sequence":"additional","affiliation":[{"name":"Microbial Biotechnology Laboratory, Department of Marine Biotechnology Bharathidasan University  Tiruchirappalli Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senthilkumar","family":"Balakrishnan","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, Tamil Nadu State Council for Science and Technology  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prabhu","family":"Subramani","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Bharathidasan University  Tiruchirappalli Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chidhambaram","family":"Prahalathan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Bharathidasan University  Tiruchirappalli Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1666-6742","authenticated-orcid":false,"given":"Anbarasu","family":"Kumarasamy","sequence":"additional","affiliation":[{"name":"Microbial Biotechnology Laboratory, Department of Marine Biotechnology Bharathidasan University  Tiruchirappalli Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,11]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.5851\/kosfa.2018.e40"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfs.12735"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104499"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034516679973"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-011-0958-1"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms151222706"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2016.08.017"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2019.06.003"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2003.10.001"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002184"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.23"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/b516237h"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-020-10989-5"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/cimb44020051"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2018.12.010"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13197-013-1214-5"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140434"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10989-019-09982-5"},{"key":"e_1_2_11_20_1","unstructured":"Jones E. 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