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However, the lack of systematic monitoring of resistance in some aquatic matrices, such as tropical estuaries, makes it unknown whether its occurrence is associated with anthropogenic pollution in these environments. Therefore, we investigated the occurrence of extended\u2010spectrum beta\u2010lactamases (ESBLs) producing\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    as a resistance indicator for 12 consecutive months at three representative points of a pollution gradient in Guanabara Bay (GB), Brazil. Sixty\u2010six\n                    <jats:italic>E. coli<\/jats:italic>\n                    strains were selected from 72 samples of GB waters in the presence of ceftriaxone (8\u2009\u03bcg mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) and identified by MALDI\u2010TOF MS. Of the 66, 55 (83.3%) strains were ESBL producers. They carried beta\u2010lactamase\/ESBL genes, with the predominance of\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M<\/jats:sub>\n                    (54, 98.2%), especially the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M\u20101,2<\/jats:sub>\n                    allele (49.1%). These strains were detected frequently (81.8%) from the point with the highest pollution levels. Furthermore, the marker for Class 1 integron,\n                    <jats:italic>intI1<\/jats:italic>\n                    gene, was detected in 54.5% of ESBL producers. These data suggest an association between antimicrobial\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    and sewage pollution in aquatic environments raising concerns about the possible risks of human exposure to these waters and fish consumption.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.16435","type":"journal-article","created":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T10:49:17Z","timestamp":1686134957000},"page":"2041-2048","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Occurrence of extended\u2010spectrum \u03b2\u2010lactamases\u2010producing\n                    <scp>\n                      <i>Escherichia coli<\/i>\n                    <\/scp>\n                    isolates over gradient pollution in an urban tropical 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Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6507-0905","authenticated-orcid":false,"given":"Renata Cristina","family":"Pic\u00e3o","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5252-0671","authenticated-orcid":false,"given":"Marinella Silva","family":"Laport","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.01972.x"},{"key":"e_1_2_8_3_1","first-page":"122","volume-title":"Master's thesis, Graduate 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