{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T16:05:43Z","timestamp":1783008343859,"version":"3.54.5"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T00:00:00Z","timestamp":1695945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100019471","name":"Mitsubishi Chemical Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019471","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Polybutylene succinate (PBS) is an eco\u2010friendly green plastic. However, PBS was shown as being non\u2010biodegradable in marine environments, and up until now, only a limited number of PBS\u2010degrading marine microbes have been discovered. We first set up in vitro PBS\u2010 and PBSA (polybutylene succinate adipate)\u2010plastispheres to characterize novel PBS\u2010degrading marine microbes. Microbial growth and oxygen consumption were observed in both PBS\u2010 and PBSA\u2010plastispheres enriched with natural seawater collected from Usujiri, Hokkaido, Japan, and\n                    <jats:italic>Vibrionaceae<\/jats:italic>\n                    and\n                    <jats:italic>Pseudoalteromonadaceae<\/jats:italic>\n                    were significantly enriched on these films. Further gene identification indicated that vibrios belonging to the Gazogenes clade possess genes related to a PBS degrading enzyme (PBSase). The PBS degradation assay for six Gazogenes clade vibrios identified\n                    <jats:italic>Vibrio ruber<\/jats:italic>\n                    ,\n                    <jats:italic>Vibrio rhizosphaerae<\/jats:italic>\n                    , and\n                    <jats:italic>Vibrio spartinae<\/jats:italic>\n                    as being capable of degrading PBS. We further identified the gene responsible for PBSase from the type strain of\n                    <jats:italic>V. ruber<\/jats:italic>\n                    , and the purified recombinant vibrio PBSase was found to have low\u2010temperature adaptation and was active under high NaCl concentrations. We also provided docking models between the vibrio PBSase and PBS and PBSA units to show how vibrio PBSase interacts with each substrate compared to the\n                    <jats:italic>Acidovorax<\/jats:italic>\n                    PBSase. These results could contribute to a more sustainable society through further utilization of PBS in marine environments and plastic recycling.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.16512","type":"journal-article","created":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T22:34:48Z","timestamp":1696026888000},"page":"2834-2850","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["A lesson from polybutylene succinate plastisphere to the discovery of novel plastic degrading enzyme genes in marine vibrios"],"prefix":"10.1111","volume":"25","author":[{"given":"Yutaro","family":"Kimura","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Faculty of Fisheries Sciences Hokkaido University  Hakodate Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Faculty of 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