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Although\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    is a recognised member of terrestrial soil microbiomes, little is known about its contribution to the saprophytic degradation of polysaccharides within its niche. For example, while chitin is predicted to be abundant within terrestrial soils the chitinolytic capacity of\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    is yet to be defined. This study identifies and characterises a putative glycoside hydrolase,\n                    <jats:italic>bpsl0500<\/jats:italic>\n                    , which is expressed by\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    K96243. Recombinant BPSL0500 was found to exhibit activity against substrate analogues and GlcNAc disaccharides relevant to chitinolytic\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetyl\u2010\u03b2\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010hexosaminidases. In\n                    <jats:italic>B. pseudomallei, bpsl0500<\/jats:italic>\n                    was found to be essential for both\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetyl\u2010\u03b2\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010hexosaminidase activity and chitooligosaccharide metabolism. Furthermore,\n                    <jats:italic>bpsl0500<\/jats:italic>\n                    was also observed to significantly affect biofilm deposition. These observations led to the identification of BPSL0500 activity against model disaccharide linkages that are present in biofilm exopolysaccharides, a feature that has not yet been described for chitinolytic enzymes. The results in this study indicate that chitinolytic\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetyl\u2010\u03b2\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010hexosaminidases like\n                    <jats:italic>bpsl0500<\/jats:italic>\n                    may facilitate biofilm disruption as well as chitin assimilation, providing dual functionality for saprophytic bacteria such as\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    within the competitive soil microbiome.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.16571","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T00:15:56Z","timestamp":1704413756000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>N<\/i>\n                    \u2010acetyl\u2010\u03b2\u2010hexosaminidase activity is important for chitooligosaccharide metabolism and biofilm formation in\n                    <i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1111","volume":"26","author":[{"given":"Clare L.","family":"Moran","sequence":"first","affiliation":[{"name":"Marshall Centre for Infectious Disease Research and Training, School of Biomedical Sciences The University of Western Australia  Nedlands Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Debowski","sequence":"additional","affiliation":[{"name":"Marshall Centre for Infectious Disease Research and Training, School of Biomedical Sciences The University of Western Australia  Nedlands Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Vrielink","sequence":"additional","affiliation":[{"name":"School of Molecular Sciences The University of Western Australia  Crawley 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