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These conditions provide a niche for chemolithoautotrophic microorganisms. Methanotrophs of the phylum\n                    <jats:italic>Verrucomicrobia<\/jats:italic>\n                    , which inhabit these ecosystems, can utilize these gases and grow at pH levels below 1 and temperatures up to 65\u00b0C. In contrast, methanotrophs of the phylum\n                    <jats:italic>Proteobacteria<\/jats:italic>\n                    are primarily found in various moderate environments. Previously, novel verrucomicrobial methanotrophs were detected and isolated from the geothermal soil of the Favara Grande on the island of Pantelleria, Italy. The detection of pmoA genes, specific for verrucomicrobial and proteobacterial methanotrophs in this environment, and the partially overlapping pH and temperature growth ranges of these isolates suggest that these distinct phylogenetic groups could coexist in the environment. In this report, we present the isolation and characterization of a thermophilic and acid\u2010tolerant gammaproteobacterial methanotroph (family\n                    <jats:italic>Methylococcaceae<\/jats:italic>\n                    ) from the Favara Grande. This isolate grows at pH values ranging from 3.5 to 7.0 and temperatures from 35\u00b0C to 55\u00b0C, and diazotrophic growth was demonstrated. Its genome contains genes encoding particulate and soluble methane monooxygenases, XoxF\u2010 and MxaFI\u2010type methanol dehydrogenases, and all enzymes of the Calvin cycle. For this novel genus and species, we propose the name \u2018\n                    <jats:italic>Candidatus<\/jats:italic>\n                    Methylocalor cossyra\u2019 CH1.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.16602","type":"journal-article","created":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T01:24:24Z","timestamp":1709861064000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Active coexistence of the novel gammaproteobacterial methanotroph \u2018\n                    <i>Ca.<\/i>\n                    <scp>Methylocalor<\/scp>\n                    cossyra\u2019\n                    <scp>CH1<\/scp>\n                    and verrucomicrobial methanotrophs in acidic, hot geothermal soil"],"prefix":"10.1111","volume":"26","author":[{"given":"Changqing","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Science Radboud Institute for Biological and Environmental Sciences, Radboud University Nijmegen  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5145-991X","authenticated-orcid":false,"given":"Rob A.","family":"Schmitz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science Radboud Institute for Biological and Environmental Sciences, Radboud University Nijmegen  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arjan","family":"Pol","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science Radboud Institute for Biological and Environmental Sciences, Radboud University Nijmegen  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Hogendoorn","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science Radboud Institute for Biological and Environmental Sciences, Radboud University Nijmegen  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dani\u00ebl","family":"Verhagen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science Radboud Institute for Biological and Environmental Sciences, Radboud University Nijmegen  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1354-8334","authenticated-orcid":false,"given":"Stijn H.","family":"Peeters","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science Radboud Institute for Biological and Environmental Sciences, Radboud University Nijmegen  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theo A.","family":"van Alen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science Radboud Institute for Biological and Environmental Sciences, Radboud University Nijmegen  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geert","family":"Cremers","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science Radboud Institute for Biological and Environmental Sciences, Radboud University Nijmegen  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0877-3676","authenticated-orcid":false,"given":"Rob A.","family":"Mesman","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Science Radboud Institute for Biological and Environmental Sciences, Radboud University Nijmegen  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1990-9030","authenticated-orcid":false,"given":"Huub J. 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