{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:09:00Z","timestamp":1790208540988,"version":"4.1.0"},"reference-count":70,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T00:00:00Z","timestamp":1724889600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T00:00:00Z","timestamp":1724889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["EAR\u20101820658"],"award-info":[{"award-number":["EAR\u20101820658"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Elemental sulfur (S\n                    <jats:sub>8<\/jats:sub>\n                    <jats:sup>0<\/jats:sup>\n                    )\u2010oxidising Sulfolobales (Archaea) dominate high\u2010temperature acidic hot springs (&gt;80\u00b0C, pH\u2009&lt;4). However, genomic analyses of S\n                    <jats:sub>8<\/jats:sub>\n                    <jats:sup>0<\/jats:sup>\n                    \u2010oxidising members of the Sulfolobales reveal a patchy distribution of genes encoding sulfur oxygenase reductase (SOR), an S\n                    <jats:sub>8<\/jats:sub>\n                    <jats:sup>0<\/jats:sup>\n                    disproportionating enzyme attributed to S\n                    <jats:sub>8<\/jats:sub>\n                    <jats:sup>0<\/jats:sup>\n                    oxidation. Here, we report the S\n                    <jats:sub>8<\/jats:sub>\n                    <jats:sup>0<\/jats:sup>\n                    \u2010dependent growth of two Sulfolobales strains previously isolated from acidic hot springs in Yellowstone National Park, one of which associated with bulk S\n                    <jats:sub>8<\/jats:sub>\n                    <jats:sup>0<\/jats:sup>\n                    during growth and one that did not. The genomes of each strain encoded different sulfur metabolism enzymes, with only one encoding SOR. Dialysis membrane experiments showed that direct contact is not required for S\n                    <jats:sub>8<\/jats:sub>\n                    <jats:sup>0<\/jats:sup>\n                    oxidation in the SOR\u2010encoding strain. This is attributed to the generation of hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) from S\n                    <jats:sub>8<\/jats:sub>\n                    <jats:sup>0<\/jats:sup>\n                    disproportionation that can diffuse out of the cell to solubilise bulk S\n                    <jats:sub>8<\/jats:sub>\n                    <jats:sup>0<\/jats:sup>\n                    to form soluble polysulfides (S\n                    <jats:sub>\n                      <jats:italic>x<\/jats:italic>\n                    <\/jats:sub>\n                    <jats:sup>2\u2212<\/jats:sup>\n                    ) and\/or S\n                    <jats:sub>8<\/jats:sub>\n                    <jats:sup>0<\/jats:sup>\n                    nanoparticles that readily diffuse across dialysis membranes. The Sulfolobales strain lacking SOR required direct contact to oxidise S\n                    <jats:sub>8<\/jats:sub>\n                    <jats:sup>0<\/jats:sup>\n                    , which could be overcome by the addition of H\n                    <jats:sub>2<\/jats:sub>\n                    S. High concentrations of S\n                    <jats:sub>8<\/jats:sub>\n                    <jats:sup>0<\/jats:sup>\n                    inhibited the growth of both strains. These results implicate alternative strategies to acquire and metabolise sulfur in Sulfolobales and have implications for their distribution and ecology in their hot spring habitats.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.16691","type":"journal-article","created":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T06:23:47Z","timestamp":1724912627000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Acquisition of elemental sulfur by sulfur\u2010oxidising Sulfolobales"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6177-4366","authenticated-orcid":false,"given":"Maria C.","family":"Fernandes\u2010Martins","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Biology Montana State University  Bozeman Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carli","family":"Springer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Montana State University  Bozeman Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3253-6833","authenticated-orcid":false,"given":"Daniel R.","family":"Colman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Montana State University  Bozeman Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4436-5856","authenticated-orcid":false,"given":"Eric S.","family":"Boyd","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Montana State University  Bozeman Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-019-02193-3"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00334-18"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14270"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2001.tb00576.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420039726"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01545-08"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1745-6150-7-12"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03160-12"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01321-07"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00408082"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.20025"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2004.04.050"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43247-022-00542-2"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-020-0611-9"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15617"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2017.162"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15189"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-7037(71)90036-6"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/pnasnexus\/pgae201"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2021.214358"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac60030a028"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2012.02898.x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-30743-5_3"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2014.07.004"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146331"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1998.tb00547.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.21775\/cimb.039.063"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gca.2009.07.003"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.3.1638-1643.1989"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.18.5854-5859.1992"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr2003006"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00154-8"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuaa063"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/93.1.28"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.768283"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2018.08.003"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.11.6106-6116.1989"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006964"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.179.4080.1323"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu300"},{"key":"e_1_2_9_42_1","first-page":"73","volume-title":"Geothermal 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