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Although sulphur\u2010rich amino acids and other substrates conducive to non\u2010sulphate\u2010based H\n                    <jats:sub>2<\/jats:sub>\n                    S production are frequently present, the capacity and potential of various microorganisms to perform sulphate\u2010free H\n                    <jats:sub>2<\/jats:sub>\n                    S production remain unclear. In this study, we identify the identity, activity and genomic characteristics of bacteria that degrade cysteine to produce H\n                    <jats:sub>2<\/jats:sub>\n                    S in anaerobic enrichment bioreactors seeded with material from aquaculture systems. Our comparison with canonical sulphate\u2010reducing bacteria reveals that both sulphur sources contribute to microbial H\n                    <jats:sub>2<\/jats:sub>\n                    S production, with cysteine facilitating a more rapid process compared to sulphate. 16S rRNA amplicon sequencing and metagenomic analysis identified four bacterial families\u2014\n                    <jats:italic>Dethiosulfatibacteraceae<\/jats:italic>\n                    ,\n                    <jats:italic>Fusobacteriaceae<\/jats:italic>\n                    ,\n                    <jats:italic>Vibrionaceae<\/jats:italic>\n                    and\n                    <jats:italic>Desulfovibrionaceae<\/jats:italic>\n                    \u2014as central to non\u2010sulphate H\n                    <jats:sub>2<\/jats:sub>\n                    S production. Metagenome\u2010 and metatranscriptome\u2010assembled genomes elucidated the primary cysteine degradation pathway mediated by cysteine desulphidase cyuA and indicated that some bacteria may also utilise cysteine as a carbon source in sulphate\u2010based H\n                    <jats:sub>2<\/jats:sub>\n                    S production.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70024","type":"journal-article","created":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T21:30:05Z","timestamp":1736371805000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identifying Bacteria Responsible for Non\u2010Sulphate\u2010Based Hydrogen Sulphide Production in Aquaculture"],"prefix":"10.1111","volume":"27","author":[{"given":"Alexandre","family":"Nguyen\u2010ti\u00eat","sequence":"first","affiliation":[{"name":"DTU Aqua, Section for Aquaculture Technical University of Denmark  Hirtshals Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Puente\u2010S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Department of Aquatic Sciences and Assessment Swedish University of Agricultural Sciences  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Bertilsson","sequence":"additional","affiliation":[{"name":"Department of Aquatic Sciences and Assessment Swedish University of Agricultural Sciences  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6724-2632","authenticated-orcid":false,"given":"Sanni L.","family":"Aalto","sequence":"additional","affiliation":[{"name":"DTU Aqua, Section for Aquaculture Technical University of Denmark  Hirtshals Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3103"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.7.4149\u20104152.2005"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016\u20107037(63)90013\u20109"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/mco.0000000000000526"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010019\u20100209\u20109"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-804564-0.00006-5"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.705583"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.2.561\u2010566.1997"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3176"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522\u2010022\u201000364\u20102"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btac672"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01936\u201022"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2021.736928"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/fermentation9020094"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01989311"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10863\u2010008\u20109166\u20106"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00411655"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0043\u20101354(85)90146\u20100"},{"key":"e_1_2_10_21_1","volume-title":"Standard Methods for the Examination of Water and Wastewater","author":"Eaton A. 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