{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T08:58:03Z","timestamp":1762851483557,"version":"build-2065373602"},"reference-count":75,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011103","name":"Rann\u00eds","doi-asserted-by":"publisher","award":["1908\u20100151"],"award-info":[{"award-number":["1908\u20100151"]}],"id":[{"id":"10.13039\/501100011103","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The specific association of the potentially plant\u2010pathogenic\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas syringae<\/jats:italic>\n                    <\/jats:styled-content>\n                    with\n                    <jats:italic>Peltigera<\/jats:italic>\n                    lichens raises questions about the factors driving this host specificity. To explore this, the metabolic profile of seven lichen species belonging to three genera (\n                    <jats:italic>Cladonia<\/jats:italic>\n                    ,\n                    <jats:italic>Peltigera<\/jats:italic>\n                    and\n                    <jats:italic>Stereocaulon<\/jats:italic>\n                    ) was analysed using LC\u2010MSMS. In addition, we assessed the growth of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. syringae<\/jats:italic>\n                    <\/jats:styled-content>\n                    strains in media supplemented with extracts from each lichen species. This revealed that\n                    <jats:italic>Peltigera<\/jats:italic>\n                    exhibits lower metabolite richness compared to other genera, but shows a higher chemical investment in specific compounds. Growth kinetics showed comparable\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. syringae<\/jats:italic>\n                    <\/jats:styled-content>\n                    growth across lichen\u2010supplemented media, except for\n                    <jats:italic>Cladonia arbuscula<\/jats:italic>\n                    and\n                    <jats:italic>Cladonia<\/jats:italic>\n                    sp., where the former exhibited lower growth rates. Inhibition assays with lichen extracts showed no inhibition of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. syringae<\/jats:italic>\n                    <\/jats:styled-content>\n                    . The lichen metabolome is predominantly composed of lipids and organic acids. Furthermore, specific compounds, such as aminoglycosides, may facilitate\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. syringae<\/jats:italic>\n                    <\/jats:styled-content>\n                    presence in\n                    <jats:italic>Peltigera<\/jats:italic>\n                    by inhibiting\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bacillus subtilis<\/jats:italic>\n                    <\/jats:styled-content>\n                    and other antagonists. In addition, compounds absent in\n                    <jats:italic>Peltigera<\/jats:italic>\n                    , like anthracene, might serve as a carbon source inhibitor like\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bacillus velezensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70057","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T21:22:59Z","timestamp":1741728179000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Exclusive Isolation of\n                    <scp>\n                      <i>Pseudomonas syringae<\/i>\n                    <\/scp>\n                    in\n                    <i>Peltigera<\/i>\n                    Lichens via Metabolite Analysis and Growth Assays"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4316-4104","authenticated-orcid":false,"given":"Natalia","family":"Ram\u00edrez","sequence":"first","affiliation":[{"name":"Department of Natural Resource Sciences University of Akureyri  Akureyri Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4158-8862","authenticated-orcid":false,"given":"Diana","family":"Vinchira\u2010Villarraga","sequence":"additional","affiliation":[{"name":"School of Biosciences and Birmingham Institute of Forest Research University of Birmingham  Birmingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8463-9087","authenticated-orcid":false,"given":"Mojgan","family":"Rabiey","sequence":"additional","affiliation":[{"name":"School of Biosciences and Birmingham Institute of Forest Research University of Birmingham  Birmingham UK"},{"name":"School of Life Sciences, Gibbet Hill Campus, University of Warwick  Coventry UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5725-2020","authenticated-orcid":false,"given":"Margr\u00e9t Au\u00f0ur","family":"Sigurbj\u00f6rnsd\u00f3ttir","sequence":"additional","affiliation":[{"name":"Department of Natural Resource Sciences University of Akureyri  Akureyri Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0329-6882","authenticated-orcid":false,"given":"Starri","family":"Heidmarsson","sequence":"additional","affiliation":[{"name":"Northwest Iceland Nature Research Centre  Sau\u00f0\u00e1rkr\u00f3kur Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8799-0964","authenticated-orcid":false,"given":"Oddur","family":"Vilhelmsson","sequence":"additional","affiliation":[{"name":"Department of Natural Resource Sciences University of Akureyri  Akureyri Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2042-4105","authenticated-orcid":false,"given":"Robert W.","family":"Jackson","sequence":"additional","affiliation":[{"name":"School of Biosciences and Birmingham Institute of Forest Research University of Birmingham  Birmingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1515\/znc\u20102013\u20105\u2010604"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-047378-9.50020-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0305\u20101978(01)00149\u20101"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0024282911000648"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10886\u2010005\u20108408\u2010x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758\u20102229.2010.00206.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1079\/PGR200572"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364\u20103703.2005.00311.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00592\u201010"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/lich.1996.0044"},{"key":"e_1_2_9_12_1","first-page":"61","article-title":"Effects of Sulfur Dioxide on Lichen Lipids and Fatty Acids","volume":"64","author":"Bychek\u2010Guschina I. A.","year":"1999","journal-title":"Biochemistry"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/c7cs00431a"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppa.12066"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574\u20106941.2006.00133.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2377"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12262"},{"volume-title":"The Lichens of Great Britain and Ireland","year":"2009","author":"Coppins B. J.","key":"e_1_2_9_18_1"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2012.07.069"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163\u20107827(92)90002\u2010Z"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/00040851.1973.12003745"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.58.030603.123730"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2017.27"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1364\u20103703.2002.00131.x"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13321\u2010016\u20100174\u2010y"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/np50072a030"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010019\u20100344\u20108"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2014.5963"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1134\/S107036321710022X"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.6b00974"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10531\u2010006\u20109142\u20106"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00139\u201016"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0024282984000293"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425\u2010003\u20101039\u2010y"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1515\/znc\u20102000\u201011\u20101208"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rechem.2022.100606"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.55.4.731"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.1c00399"},{"key":"e_1_2_9_39_1","first-page":"301","article-title":"Two Simple Media for the Demonstration of Pyocyanin and Fluorescin","volume":"44","author":"King E. O.","year":"1954","journal-title":"Journal of Laboratory and Clinical Medicine"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2015.06.006"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a027029"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248\u2010020\u201001662\u2010y"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2011.06.003"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2021.08.004"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574\u20106968.12409"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469\u20100691.12373"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1006\/lich.1997.0114"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100434.1988.tb01027.x"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12064"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1515\/znc\u20102010\u20103\u2010401"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11030357"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1127\/0029\u20105035\/2008\/0086\u20100115"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12101929"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.3390\/d11060094"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27072363"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109422(95)00322\u2010X"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2010.10.062"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24061046"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462\u20102920.16490"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.97.4.1342"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/md20060397"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-4439-2_11"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxrep.2018.11.004"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41559\u2010018\u20100552\u20100"},{"volume-title":"Molecular Cloning: A Laboratory Manual","year":"1989","author":"Sambrook J.","key":"e_1_2_9_65_1"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010023\u201001690\u20102"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2004.04.014"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.18968\/jhbl.84.0_315"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.12295"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12600\u2010019\u201000764\u20109"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf8287"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.18968\/jhbl.75.0_319"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.2104.04023"},{"key":"e_1_2_9_74_1","first-page":"217","volume-title":"Lichen Biology","author":"Tehler A.","year":"1996"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00300\u2010023\u201003168\u2010y"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI\u201003\u201011\u20100077"}],"container-title":["Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.70057","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T08:53:51Z","timestamp":1762851231000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1462-2920.70057"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":75,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1111\/1462-2920.70057"],"URL":"https:\/\/doi.org\/10.1111\/1462-2920.70057","archive":["Portico"],"relation":{},"ISSN":["1462-2912","1462-2920"],"issn-type":[{"type":"print","value":"1462-2912"},{"type":"electronic","value":"1462-2920"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"2024-09-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70057"}}