{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T10:06:03Z","timestamp":1784801163902,"version":"3.55.0"},"reference-count":66,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T00:00:00Z","timestamp":1744588800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Moorella<\/jats:italic>\n                    species are thermophilic acetogens that primarily produce acetate from one\u2010carbon (C1) compounds including CO, CO\n                    <jats:sub>2<\/jats:sub>\n                    (+H\n                    <jats:sub>2<\/jats:sub>\n                    ), methanol and formate. Notably,\n                    <jats:italic>Moorella caeni<\/jats:italic>\n                    DSM 21394\n                    <jats:sup>T<\/jats:sup>\n                    displays a hydrogenogenic metabolism on CO and an acetogenic metabolism on methanol. Furthermore,\n                    <jats:italic>M. caeni<\/jats:italic>\n                    is unable to use CO\n                    <jats:sub>2<\/jats:sub>\n                    (+H\n                    <jats:sub>2<\/jats:sub>\n                    ) and grows only on formate in the presence of a methanogen or when thiosulfate is added as an electron acceptor. Presently, all theoretical frameworks for C1 metabolism in\n                    <jats:italic>Moorella<\/jats:italic>\n                    species are derived from experimental and genomic analyses of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Moorella thermoacetica<\/jats:italic>\n                    <\/jats:styled-content>\n                    , which exhibits an acetogenic metabolism with all C1 substrates. In this study, we applied a transcriptomics approach to elucidate the mechanisms underlying the C1 metabolism of\n                    <jats:italic>Moorella caeni<\/jats:italic>\n                    during growth on methanol and CO. Our results indicate that respiratory Complex 1, a proton\u2010translocating (ubi)quinone oxidoreductase, is the primary respiratory enzyme in methanol\u2010grown cells of\n                    <jats:italic>M. caeni<\/jats:italic>\n                    . Conversely, in CO\u2010grown cells, an energy\u2010conserving hydrogenase complex (Ech) appears to be the primary respiratory complex, alongside respiratory Complex 1. This study provides insight into the C1 metabolism of\n                    <jats:italic>M. caeni<\/jats:italic>\n                    and reveals variations in gene syntenies related to C1 metabolism among the\n                    <jats:italic>Moorella<\/jats:italic>\n                    genus.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70096","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T18:33:56Z","timestamp":1744655636000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Methanol and Carbon Monoxide Metabolism of the Thermophile\n                    <i>Moorella caeni<\/i>"],"prefix":"10.1111","volume":"27","author":[{"given":"Nicolas A.","family":"Vecchini Santaella","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology Wageningen University &amp; Research  Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfons J. 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