{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T02:28:43Z","timestamp":1785292123568,"version":"3.55.0"},"reference-count":72,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T00:00:00Z","timestamp":1754179200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["ERC Advanced Grant VOLCANO 669371"],"award-info":[{"award-number":["ERC Advanced Grant VOLCANO 669371"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["ERC Synergy Grant MARIX 854088"],"award-info":[{"award-number":["ERC Synergy Grant MARIX 854088"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hydrogen (H\n                    <jats:sub>2<\/jats:sub>\n                    ) and methane (CH\n                    <jats:sub>4<\/jats:sub>\n                    ) are produced in the anoxic layers of wetlands and sediments. In the overlaying oxygenated surface layers, these gases become available for oxidation by aerobic hydrogenotrophic and methanotrophic microorganisms. While H\n                    <jats:sub>2<\/jats:sub>\n                    oxidation by verrucomicrobial methane\u2010oxidising bacteria (MOB) is extensively studied, less is known about this metabolism in MOB from the class\n                    <jats:italic>Alphaproteobacteria<\/jats:italic>\n                    , which frequently inhabit wetlands. We show that\n                    <jats:italic>Methylocystis bryophila<\/jats:italic>\n                    H2s\n                    <jats:sup>T<\/jats:sup>\n                    ,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Methylocapsa aurea<\/jats:italic>\n                    <\/jats:styled-content>\n                    KYG\n                    <jats:sup>T<\/jats:sup>\n                    , and \u201c\n                    <jats:italic>Methylosinus acidophilus<\/jats:italic>\n                    \u201d 29 encode diverse hydrogenases, instantly oxidise H\n                    <jats:sub>2<\/jats:sub>\n                    when cultivated under CH\n                    <jats:sub>4<\/jats:sub>\n                    \u2010limited and low\u2010oxygen conditions, under which hydrogenase transcription is upregulated compared to CH\n                    <jats:sub>4<\/jats:sub>\n                    \u2010replete conditions. H\n                    <jats:sub>2<\/jats:sub>\n                    exposure accelerated the maximum H\n                    <jats:sub>2<\/jats:sub>\n                    oxidation rates but caused no upregulation of hydrogenases. Furthermore, while CH\n                    <jats:sub>4<\/jats:sub>\n                    oxidation activity was affected by substrate\u2010limited growth conditions, H\n                    <jats:sub>2<\/jats:sub>\n                    oxidation rates remained unaffected, and H\n                    <jats:sub>2<\/jats:sub>\n                    supply to CH\n                    <jats:sub>4<\/jats:sub>\n                    \u2010limited chemostats caused increased biomass yield. Moreover, CH\n                    <jats:sub>4<\/jats:sub>\n                    oxidation was severely inhibited by sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S), while H\n                    <jats:sub>2<\/jats:sub>\n                    and methanol oxidation rates were only moderately affected. In summary, the ability to conserve energy from H\n                    <jats:sub>2<\/jats:sub>\n                    oxidation increases resilience and enhances growth of alphaproteobacterial methanotrophs in CH\n                    <jats:sub>4<\/jats:sub>\n                    \u2010limited environments, which revises the ecological role of these MOB in ecosystems with naturally fluctuating CH\n                    <jats:sub>4<\/jats:sub>\n                    and H\n                    <jats:sub>2<\/jats:sub>\n                    concentrations.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70163","type":"journal-article","created":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T04:54:13Z","timestamp":1754283253000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Hydrogen Oxidation Benefits Alphaproteobacterial Methanotrophs Under Severe Methane Limitation"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2600-2166","authenticated-orcid":false,"given":"Ida F.","family":"Peterse","sequence":"first","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"},{"name":"Department of Ecology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8950-7843","authenticated-orcid":false,"given":"Arjan","family":"Pol","sequence":"additional","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2460-6552","authenticated-orcid":false,"given":"Geert","family":"Cremers","sequence":"additional","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1833-8731","authenticated-orcid":false,"given":"Tom","family":"Berben","sequence":"additional","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5602-3937","authenticated-orcid":false,"given":"Theo A.","family":"van Alen","sequence":"additional","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1990-9030","authenticated-orcid":false,"given":"Huub J. M.","family":"Op den Camp","sequence":"additional","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6286-7484","authenticated-orcid":false,"given":"Annelies J.","family":"Veraart","sequence":"additional","affiliation":[{"name":"Department of Ecology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2935-4454","authenticated-orcid":false,"given":"Sebastian","family":"L\u00fccker","sequence":"additional","affiliation":[{"name":"Department of Microbiology Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3103"},{"key":"e_1_2_11_3_1","unstructured":"Andrews S.2010.\u201cA Quality Control Tool for High Throughput Sequence Data.\u201dhttps:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btaf147"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00034-07"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758-2229.2010.00180.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.043505-0"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12320"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009802200"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20140082"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3519"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2003.03933.x"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M500601200"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.9.3122-3132.2005"},{"key":"e_1_2_11_16_1","volume-title":"BBMap: A Fast, Accurate, Splice\u2010Aware Aligner","author":"Bushnell B.","year":"2014"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp348"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2017.112"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1029\/1999GB001221"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btac672"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20236-7"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.091208.073600"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-01940-w"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbinf.2022.861505"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msz189"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0026261709060010"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.020149-0"},{"key":"e_1_2_11_28_1","doi-asserted-by":"crossref","unstructured":"Echeveste Medrano M. 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