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Cu in the drainage was rapidly removed through Fe(II) oxidation and subsequent Fe(III) biomineralisation. The resulting Fe(III) biomineral\u2010rich sediment contained up to ~2\u2009wt% Cu, a percent\u2010level high content. XAFS and XRD analyses directly demonstrated that Cu was predominantly associated with poorly crystalline Fe(III) oxyhydroxides. The microbial community in the drainage was dominated by chemolithotrophic Fe(II)\u2010oxidising bacteria of family\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Gallionellaceae<\/jats:italic>\n                    <\/jats:styled-content>\n                    (33.8%), suggesting their key role in Cu and Fe biomineralisation. Using a custom\u2010made medium,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Gallionellaceae<\/jats:italic>\n                    <\/jats:styled-content>\n                    bacteria were enriched and a representative strain TK5 was isolated. 16S rRNA gene analysis revealed that TK5 was almost identical to the most abundant taxon at the site and likely represents a novel species of genus\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Sideroxyarcus<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Physiological and genomic analyses confirmed its Fe(II) oxidation ability and a relatively high Cu(II) tolerance (up to 50\u2009\u03bcM), which is about 20\u2010fold higher than on\u2010site Cu concentration. Strain TK5 is the first known Cu\u2010tolerant Fe(II)\u2010oxidising\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Gallionellaceae<\/jats:italic>\n                    <\/jats:styled-content>\n                    isolate. Batch experiments demonstrated that TK5 effectively promoted Cu deposition via Fe(III) biomineralisation. This study highlights the potential of Fe(II)\u2010oxidising bacteria for Cu bioremediation and biomineralisation under circumneutral\u2010pH conditions.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70212","type":"journal-article","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T04:54:58Z","timestamp":1764564898000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Percent\u2010Level Copper Mineralisation Promoted by Copper\u2010Tolerant Iron\u2010Oxidising Bacteria in Circumneutral Mine Drainage"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4655-5288","authenticated-orcid":false,"given":"Kazuya","family":"Tanimoto","sequence":"first","affiliation":[{"name":"Department of Science and Technology for Biological Resources and Environment, Graduate School of Agriculture Ehime University  Matsuyama Ehime Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4175-0532","authenticated-orcid":false,"given":"Shingo","family":"Kato","sequence":"additional","affiliation":[{"name":"Japan Collection of Microorganisms, RIKEN BioResource Research Center  Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7555-3548","authenticated-orcid":false,"given":"Kohei","family":"Tokunaga","sequence":"additional","affiliation":[{"name":"Ningyo\u2010Toge Environmental Engineering Center, Japan Atomic Energy Agency  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6641-1975","authenticated-orcid":false,"given":"Natsuko","family":"Hamamura","sequence":"additional","affiliation":[{"name":"Department of Biology, Graduate School of Science Kyushu University  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3328-8245","authenticated-orcid":false,"given":"Moriya","family":"Ohkuma","sequence":"additional","affiliation":[{"name":"Japan Collection of Microorganisms, RIKEN BioResource Research Center  Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8713-9368","authenticated-orcid":false,"given":"Satoshi","family":"Mitsunobu","sequence":"additional","affiliation":[{"name":"Department of Science and Technology for Biological Resources and Environment, Graduate School of Agriculture Ehime University  Matsuyama Ehime Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2007.34"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.proenv.2012.03.002"},{"key":"e_1_2_10_4_1","first-page":"1","article-title":"Mechanisms of Copper Homeostasis in Bacteria","volume":"4","author":"Arg\u00fcello J. 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