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Keck Foundation","doi-asserted-by":"publisher","award":["MSU D19"],"award-info":[{"award-number":["MSU D19"]}],"id":[{"id":"10.13039\/100000888","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Acquisition and cycling of carbon and nitrogen among members of hot spring communities are not well understood. Metagenomic analyses of 105 communities inhabiting high temperature hot springs across Yellowstone and Iceland showed a co\u2010distribution pattern of putatively autotrophic and\/or diazotrophic (nitrogen\u2010fixing) Aquificales and\n                    <jats:italic>Thermus<\/jats:italic>\n                    populations. Targeted enrichment of autotrophic and diazotrophic populations in an Icelandic hot spring produced a co\u2010culture of\n                    <jats:italic>Pampinifervens<\/jats:italic>\n                    (Aquificales) that encoded carbon dioxide and nitrogen fixation pathways and\n                    <jats:italic>Thermus<\/jats:italic>\n                    (Thermales). Growth experiments revealed\n                    <jats:italic>Pampinifervens<\/jats:italic>\n                    could support the fixed carbon and nitrogen demands of\n                    <jats:italic>Thermus<\/jats:italic>\n                    , enabling growth. Interestingly, growth of\n                    <jats:italic>Thermus<\/jats:italic>\n                    was enhanced in co\u2010cultures when\n                    <jats:italic>Pampinifervens<\/jats:italic>\n                    was forced to fix both carbon and nitrogen versus just carbon (ammonia\u2010amended cultures). Further experimentation with\n                    <jats:italic>Thermus<\/jats:italic>\n                    , when grown in isolation, showed it preferred amino acids over ammonia as a nitrogen source. These findings demonstrate the importance of metabolic interactions among populations that can dictate the co\u2010distribution of taxa in hot springs, drive community assembly, and maintain biodiversity. Further, these results highlight the fundamental role of Aquificales in the functioning of hot spring ecosystems, particularly those limited in organic carbon and fixed nitrogen like those in Iceland and to a lesser extent Yellowstone.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70225","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:06:44Z","timestamp":1767856004000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cross\u2010Feeding of Carbon and Nitrogen Between Aquificales and\n                    <i>Thermus<\/i>\n                    in Hot Springs"],"prefix":"10.1111","volume":"28","author":[{"given":"Lisa M.","family":"Keller","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Biology Montana State University  Bozeman Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3253-6833","authenticated-orcid":false,"given":"Daniel R.","family":"Colman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Montana State University  Bozeman Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andri","family":"Stef\u00e1nsson","sequence":"additional","affiliation":[{"name":"Institute of Earth Sciences University of Iceland  Reykjavik Iceland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4436-5856","authenticated-orcid":false,"given":"Eric S.","family":"Boyd","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Montana State University  Bozeman Montana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02790-0"},{"key":"e_1_2_10_3_1","first-page":"1","volume-title":"Bergey's Manual of Systematics of Archaea and Bacteria","author":"Albuquerque L.","year":"2014"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2001.tb00576.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0375-6505(95)00025-9"},{"key":"e_1_2_10_6_1","unstructured":"\u00c1sgeirsd\u00f3ttir R. 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