{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T13:53:19Z","timestamp":1788443599896,"version":"build-2803163510"},"reference-count":75,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009171","name":"Natural Resources Conservation Service","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Highbush blueberry (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Vaccinium corymbosum<\/jats:italic>\n                    <\/jats:styled-content>\n                    L.) fields can remain productive for decades. However, some older fields decline in plant health and exhibit lower yields. After re\u2010planting with new stock, the yields continue to suffer. This condition is termed \u2018Replant Disease\u2019. The causative agent(s) in replant disease in New Jersey blueberry fields are unknown. To assess if low\u2010 and high\u2010yield blueberry farm soils from two separate farms contained different microbiomes, we coupled long\u2010read bacterial and eukaryotic ribosomal rRNA operon sequencing using the Oxford Nanopore MinION with stable isotope probing (SIP) to detect\n                    <jats:sup>13<\/jats:sup>\n                    C\/\n                    <jats:sup>15<\/jats:sup>\n                    N\u2010utilising soil microbial communities. The results indicate multiple\n                    <jats:italic>Bacillus<\/jats:italic>\n                    species were active on\n                    <jats:sup>13<\/jats:sup>\n                    C\/\n                    <jats:sup>15<\/jats:sup>\n                    N\u2010growth media (predominantly amino acids and glucose) in low\u2010productivity soils from both farms, while high\u2010productivity and adjacent forest soils contained active\n                    <jats:italic>Burkholderia<\/jats:italic>\n                    and\n                    <jats:italic>Paraburkholderia<\/jats:italic>\n                    species. Eukaryotic community profiling indicated\n                    <jats:italic>Candida blankii<\/jats:italic>\n                    ,\n                    <jats:italic>Nadsonia starkeyi<\/jats:italic>\n                    and\n                    <jats:italic>Sugiyamaella chiloensis<\/jats:italic>\n                    were slightly enriched and active in low\u2010productivity soils compared with high\u2010productivity soils. This approach differentiates low\u2010 and high\u2010productivity blueberry farm soils by ribosomal RNA operon profiling and SIP. The findings also suggest a diagnostic test of blueberry replant affected soils is feasible and may ultimately be used to improve productivity and potentially detect the responsible pathogenic agent(s) or other deleterious microbes.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70280","type":"journal-article","created":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T14:32:48Z","timestamp":1773757968000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["High\u2010Resolution Community Profiling of Active Bacteria and Eukaryotes in Replant\u2010Diseased Blueberry Farm Soils From New Jersey,\n                    <scp>USA<\/scp>"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4884-7422","authenticated-orcid":false,"given":"Seda","family":"Mirzoyan","sequence":"first","affiliation":[{"name":"Microbial Biology Graduate Program Rutgers, The State University of New Jersey  New Brunswick New Jersey USA"},{"name":"Department of Zoology, Faculty of Science Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7977-2921","authenticated-orcid":false,"given":"James J.","family":"Polashock","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture\u2010Agricultural Research Service Genetic Improvement for Fruits &amp; Vegetables Laboratory  Chatsworth New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9107-8695","authenticated-orcid":false,"given":"Lee J.","family":"Kerkhof","sequence":"additional","affiliation":[{"name":"Department of Marine and Coastal Sciences Rutgers, The State University of New Jersey  New Brunswick New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2004.tb00295.x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjm\u20102022\u20100203"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1264\/jsme2.ME16011"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/gix043"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.174333"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2008.08.007"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-014-4138-8"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-014-3838-4"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.16817.1"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/biomethods\/bpaa026"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41426\u2010017\u20100015\u20108"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00487.2023"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-65740-4"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1163\/15685411-00003345"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1590\/0100-2945-128\/13"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiz192"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.9.5192-5196.2005"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02274-09"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1071\/SR19204"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1094\/PBIOMES-10-22-0071-R"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43008-019-0006-6"},{"key":"e_1_2_11_23_1","first-page":"143","article-title":"Soil Requirements of Blueberries in Relation to their Nutrition","volume":"18","author":"Hayes R. 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