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Members of the genera\n                    <jats:italic>Thermincola<\/jats:italic>\n                    and\n                    <jats:italic>Carboxydocella<\/jats:italic>\n                    are capable of CO oxidation via the water\u2010gas shift reaction, generating H\n                    <jats:sub>2<\/jats:sub>\n                    , but comprise only two and three validly described species, respectively. In this study, we report the isolation of two novel bacterial strains: strain AZ34E, affiliated with\n                    <jats:italic>Thermincola<\/jats:italic>\n                    , and strain AZ29I, affiliated with\n                    <jats:italic>Carboxydocella<\/jats:italic>\n                    . Comparative genomic and phylogenetic analyses revealed that all currently described isolates within\n                    <jats:italic>Thermincola<\/jats:italic>\n                    and\n                    <jats:italic>Carboxydocella<\/jats:italic>\n                    each represent a single species within their respective genera.\n                    <jats:italic>Thermincola<\/jats:italic>\n                    genomes contain four gene copies encoding [Ni\u2013Fe] CO dehydrogenases, while\n                    <jats:italic>Carboxydocella<\/jats:italic>\n                    genomes harbour six copies with diverse predicted functional roles, suggesting high metabolic flexibility for CO oxidation in these thermophiles. The isolation of the two novel strains, both members of the Bacillota_B phylum, motivated a broader genomic survey across this lineage. This search uncovered numerous candidate organisms with genomic potential for CO oxidation, substantially expanding the diversity of putative carboxydotrophs. These findings highlight the value of targeted genomic mining approaches, which may reveal a much wider array of CO\u2010oxidising microorganisms than currently recognised.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70283","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T08:25:36Z","timestamp":1773908736000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative Physiology and Genomics of\n                    <i>Thermincola<\/i>\n                    and\n                    <i>Carboxydocella<\/i>\n                    Strains and Description of Two Novel Isolates"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0533-6600","authenticated-orcid":false,"given":"Anastasia","family":"Galani","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology Wageningen University &amp; Research  Wageningen the 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