{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T10:06:01Z","timestamp":1784801161500,"version":"3.55.0"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Although the Wood\u2010Ljungdahl pathway, a wide\u2010spread pathway for CO\n 2<\/jats:sub>\n fixation in anaerobic microorganisms, was elucidated in the thermophilic acetogenic bacterium\n \n Moorella thermoacetica<\/jats:italic>\n <\/jats:styled-content>\n , still relatively little is known about the enzymes involved in hydrogen oxidation, CO\n 2<\/jats:sub>\n fixation, energy conservation and the role of quinones and cytochromes. Here, we have used transcriptomics, enzyme assays and genome analyses to identify missing links. NADPH, generated by a [FeFe] hydrogenase, is the reductant for CO\n 2<\/jats:sub>\n reduction to formate, a key reaction in CO\n 2<\/jats:sub>\n fixation. This reaction is slightly endergonic under standard conditions but becomes thermodynamically feasible at high environmental H\n 2<\/jats:sub>\n concentrations. In addition, formate is taken out of equilibrium by a formate dehydrogenase that potentially forms a complex with an energy\u2010converting hydrogenase (Fdh\u2013Ech), a novel respiratory enzyme in acetogens. Under low H\n 2<\/jats:sub>\n concentrations, the complex can drive the reverse, endergonic reaction. In addition, we postulate a formate cycle involving a periplasmic, cytochrome\n b<\/jats:italic>\n \u2010containing formate dehydrogenase. A NADH dehydrogenase\u2010like enzyme that uses reduced ferredoxin instead of NADH to reduce menaquinone is also involved in energy conservation. The data are summarised in a comprehensive metabolic and bioenergetic model of acetogenesis from H\n 2<\/jats:sub>\n \u2009+\u2009CO\n 2<\/jats:sub>\n and CO in\n \n M. thermoacetica<\/jats:italic>\n <\/jats:styled-content>\n .\n <\/jats:p>","DOI":"10.1111\/1462-2920.70289","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T08:31:54Z","timestamp":1775032314000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genomic and Transcriptomic Insights Into the Autotrophic Metabolism on\n \n H\n 2<\/sub>\n <\/scp>\n \u2009+\u2009\n \n CO\n 2<\/sub>\n <\/scp>\n or\n CO<\/scp>\n of the Thermophilic Acetogenic Model Organism\n \n Moorella thermoacetica<\/i>\n <\/scp>"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6912-1981","authenticated-orcid":false,"given":"Florian P.","family":"Rosenbaum","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences Johann Wolfgang Goethe University Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2473-6202","authenticated-orcid":false,"given":"Anja","family":"Poehlein","sequence":"additional","affiliation":[{"name":"Genomic and Applied Microbiology & G\u00f6ttingen Genomics Laboratory Georg\u2010August University G\u00f6ttingen G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8646-7925","authenticated-orcid":false,"given":"Rolf","family":"Daniel","sequence":"additional","affiliation":[{"name":"Genomic and Applied Microbiology & G\u00f6ttingen Genomics Laboratory Georg\u2010August University G\u00f6ttingen G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences Johann Wolfgang Goethe University Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,1]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.03740-21","article-title":"Enoyl\u2010Coenzyme A Respiration via Formate Cycling in Syntrophic Bacteria","volume":"13","author":"Agne M.","year":"2021","journal-title":"MBio"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2010.10.017"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000650200"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00555-9"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13082"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/25.12.1324"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)34857-9"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15643"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1419.016"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)68568-6"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2009-0026"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00385-12"},{"key":"e_1_2_10_14_1","first-page":"117","volume-title":"Methods in Microbiology","author":"Hungate R. 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