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The aim of this study was to explore the variety of gut microbes, metabolites and trace elements in the whole life cycle (from occurrence to metastasis to cachexia) of CRC. Stool samples from 302 volunteers (107 CRC, 69 adenoma dvanced, 126 controls) underwent DNA extraction and full\u2010length 16S rDNA sequencing (PacBio Sequel II). Gut metabolites and trace elements were analysed by mass spectrometry to assess CRC\u2010associated variations. The results found that a total of seven bacteria (\n                    <jats:italic>\n                      Peptostreptococcus\n                      <jats:styled-content style=\"fixed-case\">anaerobius<\/jats:styled-content>\n                    <\/jats:italic>\n                    , etc.), one trace element (Plumbum) and four metabolites (VLH, etc.) could be used as biomarkers to construct CRC identification models with an accuracy of 93.3%. Metastatic CRC showed distinct microbial\/metabolite profiles (10 bacteria such as\n                    <jats:italic>Faecalibacterium_prausnitzii_G<\/jats:italic>\n                    and 19 metabolites such as adrenic acid), while\n                    <jats:italic>Lachnotalea soehngenii<\/jats:italic>\n                    , PD 0200347 and Vanadium predicted malnutrition risk (80% accuracy). In conclusion, gut bacteria, metabolites and trace elements serve as predictive biomarkers for CRC risk, metastasis and malnutrition while supporting therapeutic drug research.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70292","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T02:11:48Z","timestamp":1775527908000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi\u2010Omics Analysis of Gut Microecological Variations in Colorectal Cancer"],"prefix":"10.1111","volume":"28","author":[{"given":"Wu","family":"Yinhang","sequence":"first","affiliation":[{"name":"Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University  Huzhou Zhejiang People's Republic of China"},{"name":"Zhejiang\u2010France United Laboratory of Integrated Traditional Chinese and Modern Medicine in Colorectal Cancer  Huzhou Zhejiang People's Republic of China"},{"name":"ASIR (Institute\u2014Association of Intelligent Systems and Robotics)  Rueil\u2010Malmaison France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6910-8824","authenticated-orcid":false,"given":"Zhuang","family":"Jing","sequence":"additional","affiliation":[{"name":"Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University  Huzhou Zhejiang People's Republic of China"},{"name":"Zhejiang\u2010France United Laboratory of Integrated Traditional Chinese and Modern Medicine in Colorectal Cancer  Huzhou Zhejiang People's Republic of China"},{"name":"ASIR (Institute\u2014Association of Intelligent Systems and Robotics)  Rueil\u2010Malmaison France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chu","family":"Jian","sequence":"additional","affiliation":[{"name":"Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University  Huzhou Zhejiang People's Republic of China"},{"name":"Zhejiang\u2010France United Laboratory of Integrated Traditional Chinese and Modern Medicine in Colorectal Cancer  Huzhou Zhejiang People's Republic of China"},{"name":"ASIR (Institute\u2014Association of Intelligent Systems and Robotics)  Rueil\u2010Malmaison France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Zhiqin","sequence":"additional","affiliation":[{"name":"Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University  Huzhou Zhejiang People's Republic of China"},{"name":"Zhejiang\u2010France United Laboratory of Integrated Traditional Chinese and Modern Medicine in Colorectal Cancer  Huzhou Zhejiang People's Republic of China"},{"name":"ASIR (Institute\u2014Association of Intelligent Systems and Robotics)  Rueil\u2010Malmaison France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Xiaojian","sequence":"additional","affiliation":[{"name":"Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University  Huzhou Zhejiang People's Republic of China"},{"name":"Zhejiang\u2010France United Laboratory of Integrated Traditional Chinese and Modern Medicine in Colorectal Cancer  Huzhou Zhejiang People's Republic of China"},{"name":"ASIR (Institute\u2014Association of Intelligent Systems and Robotics)  Rueil\u2010Malmaison France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wang","family":"Zefeng","sequence":"additional","affiliation":[{"name":"ASIR (Institute\u2014Association of Intelligent Systems and Robotics)  Rueil\u2010Malmaison France"},{"name":"School of Information Engineering Huzhou University  Huzhou Zhejiang People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wang","family":"Yingchen","sequence":"additional","affiliation":[{"name":"Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University  Huzhou Zhejiang People's Republic of China"},{"name":"Zhejiang\u2010France United Laboratory of Integrated Traditional Chinese and Modern Medicine in Colorectal Cancer  Huzhou Zhejiang People's Republic of China"},{"name":"ASIR (Institute\u2014Association of Intelligent Systems and Robotics)  Rueil\u2010Malmaison France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Peng","sequence":"additional","affiliation":[{"name":"Hangzhou Black Box Biotechnology Co. 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