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We investigated bacterioplankton communities along the northwest Congo watershed using 16S rRNA and metagenomic sequencing, paired with hydrological, biogeochemical, and greenhouse gas data. In large rivers, community composition correlated with temperature and water travel time, while smaller streams were shaped by nutrient chemistry and landscape. Most sites were dominated by Burkholderiales, but composition varied, especially in DOC\u2010rich Cuvette Centrale wetland streams that hosted distinct communities associated with high methane and CO\n                    <jats:sub>2<\/jats:sub>\n                    , and low oxygen. Indicator species analysis identified specific taxa and metagenome\u2010assembled genomes (MAGs) strongly associated with long travel times, wetlands, and methane, including methanotrophs (Methylcoccaceae, Methylophilaceae, Methylomonas) and MAGs encoding diverse carbon\u2010processing metabolisms. 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The striking similarity of tropical river bacterioplankton from Africa and Asia suggests the primacy of environmental controls on river bacterioplankton.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70327","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T22:22:23Z","timestamp":1779402143000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Congo River Bacterioplankton Genomic Diversity Reflects Water Travel Time, Wetland Habitats, and Greenhouse Gases"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8452-3500","authenticated-orcid":false,"given":"Ted","family":"Bambakidis","sequence":"first","affiliation":[{"name":"Department of Microbiology Oregon State University  Corvallis Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0836-5085","authenticated-orcid":false,"given":"Shaoda","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Water and Sediment Sciences of Ministry of Education &amp; State Key Laboratory of Wetland Conservation and Restoration, School of Environment Beijing Normal University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5073-7456","authenticated-orcid":false,"given":"Alexandra M.","family":"Wettengel","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6413-9154","authenticated-orcid":false,"given":"R. 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