{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T10:19:25Z","timestamp":1789553965845,"version":"build-2803163510"},"reference-count":72,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"publisher","award":["ED431C 2024\/22"],"award-info":[{"award-number":["ED431C 2024\/22"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000893","name":"Simons Foundation","doi-asserted-by":"publisher","award":["877215"],"award-info":[{"award-number":["877215"]}],"id":[{"id":"10.13039\/100000893","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In the last decade, numerous laboratory experiments have demonstrated that when marine phytoplankton are exposed to thermal stress, they can evolve high temperature tolerance in a short time (months). This evolutionary potential may ensure the persistence of marine phytoplankton species under current and future global warming. However, the effect of such adaptation on the phytoplankton's interaction with the environment and other organisms depends on how cellular metabolism shifts during the evolutionary process. In order to elucidate which cellular strategies allow the emergence of thermo\u2010tolerant lines, we analysed the proteomic changes in a marine diatom (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Chaetoceros simplex<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) following both thermal acclimation and evolutionary adaptation. We found that the high temperature\u2010tolerant lines exhibit a conservative cellular strategy when acclimated to high, above\u2010optimal temperature, where nitrogen recycling, reallocation of carbon and nitrogen, and protein repair or protection are favoured at the expense of new proteins and photosynthetic structures biosynthesis and rapid growth. While this strategy gives the lines that evolved high temperature tolerance an advantage under thermal stress, the shift to resource reallocation may explain the absence of high\u2010temperature adaptation when cells are exposed to low nitrate availability.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70341","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T12:17:43Z","timestamp":1781007463000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Metabolic Strategies Under Heat Stress in Non\u2010Adapted and High\u2010Temperature\u2010Adapted Lines of a Marine Diatom"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7521-3348","authenticated-orcid":false,"given":"Mar\u00eda","family":"Aranguren\u2010Gassis","sequence":"first","affiliation":[{"name":"Grupo de Oceanograf\u00eda Biol\u00f3xica (GOB), Departamento de Ecolox\u00eda e Biolox\u00eda Animal Centro de Investigaci\u00f3n Mari\u00f1a (CIM), Universidade de Vigo  Vigo Spain"},{"name":"W. 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Kellogg Biological Station, Michigan State University  Hickory Corners Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8426-4166","authenticated-orcid":false,"given":"Angel P.","family":"Diz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Genetics and Immunology Centro de Investigaci\u00f3n Mari\u00f1a (CIM), Universidade de Vigo  Vigo Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7819-5851","authenticated-orcid":false,"given":"Suzana G.","family":"Leles","sequence":"additional","affiliation":[{"name":"Department of Marine and Environmental Biology University of Southern California  Los Angeles California USA"},{"name":"Department of Marine Science The University of Texas at Austin, Marine Science Institute  Port Aransas Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8746-5518","authenticated-orcid":false,"given":"Mar\u00eda","family":"Huete\u2010Ortega","sequence":"additional","affiliation":[{"name":"Department of Plant Science University of Cambridge  Cambridge UK"},{"name":"Centro de Investigaci\u00f3n Mari\u00f1a (CIM), Universidade de Vigo  Vigo Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5911-6081","authenticated-orcid":false,"given":"Andrew","family":"Allen","sequence":"additional","affiliation":[{"name":"Scripps Institution of Oceanography, University of California  San Diego, La Jolla California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4963-0535","authenticated-orcid":false,"given":"Naomi M.","family":"Levine","sequence":"additional","affiliation":[{"name":"Department of Marine and Environmental Biology University of Southern California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7736-6332","authenticated-orcid":false,"given":"Elena","family":"Litchman","sequence":"additional","affiliation":[{"name":"W. 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Kellogg Biological Station, Michigan State University  Hickory Corners Michigan USA"},{"name":"Department of Integrative Biology Michigan State University  East Lansing Michigan USA"},{"name":"Ecology, Evolution and Behavior Program, Michigan State University  East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10074"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ele.13378"},{"issue":"6","key":"e_1_2_10_4_1","first-page":"680","article-title":"Thermal Performance of Marine Diatoms Under Contrasting Nitrate Availability","volume":"42","author":"Aranguren\u2010Gassis M.","year":"2020","journal-title":"Journal of Plankton Research"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2016.00044"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1519080113"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/ele.13469"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-012-9305-8"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nclimate2811"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-209"},{"key":"e_1_2_10_12_1","unstructured":"Castro\u2010Conde I. andJ.deU\u00f1a\u2010\u00c1lvarez.2022.Sgof: Multiple Hypothesis Testing. 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