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Acetogens use the Wood\u2010Ljungdahl pathway to disproportionate methanol to CO\n                    <jats:sub>2<\/jats:sub>\n                    and acetate. Methanol metabolism has been studied primarily in mesophilic acetogens such as\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Acetobacterium woodii<\/jats:italic>\n                    <\/jats:styled-content>\n                    and\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">Eubacterium callanderi<\/jats:styled-content>\n                      ,\n                    <\/jats:italic>\n                    that do not have cytochromes or quinones.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Moorella thermoacetica<\/jats:italic>\n                    <\/jats:styled-content>\n                    , the model organism for elucidation of acetogenesis, is a thermophilic, cytochrome\u2010 and quinone\u2010containing acetogen which is commonly found in terrestrial soil. Here, we elucidated the physiology and bioenergetics of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. thermoacetica<\/jats:italic>\n                    <\/jats:styled-content>\n                    growing on methanol by performing physiological and biochemical experiments as well as genome\u2010wide transcription analysis. Thereby, we identified genes and enzymes involved in acetogenesis from methanol. We will present a comprehensive model for carbon and energy flow from methanol to acetate and line out the bioenergetics of methanol\u2010based acetogenesis in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. thermoacetica<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Furthermore, we show that in the presence of DMSO as an alternative electron sink, no acetate is produced from methanol.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70377","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T22:19:22Z","timestamp":1784758762000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Methanol Metabolism in the Cytochrome and Menaquinone\u2010Containing Acetogen\n                    <scp>\n                      <i>Moorella thermoacetica<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6912-1981","authenticated-orcid":false,"given":"Florian P.","family":"Rosenbaum","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology &amp; Bioenergetics, Institute of Molecular Biosciences Johann Wolfgang Goethe University  Frankfurt am Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2473-6202","authenticated-orcid":false,"given":"Anja","family":"Poehlein","sequence":"additional","affiliation":[{"name":"Genomic and Applied Microbiology &amp; G\u00f6ttingen Genomics Laboratory Georg\u2010August University G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8646-7925","authenticated-orcid":false,"given":"Rolf","family":"Daniel","sequence":"additional","affiliation":[{"name":"Genomic and Applied Microbiology &amp; G\u00f6ttingen Genomics Laboratory Georg\u2010August University G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volker","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology &amp; Bioenergetics, Institute of Molecular Biosciences Johann Wolfgang Goethe University  Frankfurt am Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2021.148379"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2025.103354"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00048-15"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13082"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010318107"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/25.12.1324"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)34857-9"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)81309-9"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.21094"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.11.5823-5829.1989"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15643"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1419.016"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)68568-6"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00203a018"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2016978118"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603650103"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00385-12"},{"key":"e_1_2_10_20_1","first-page":"117","volume-title":"Methods in Microbiology","author":"Hungate R. 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