{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T06:25:17Z","timestamp":1787639117077,"version":"build-2736575974"},"reference-count":55,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The rapid evolution of pesticide resistance in\n                    <jats:italic>Plutella xylostella<\/jats:italic>\n                    has reduced the effectiveness of conventional pest control methods in cruciferous crops. This study investigated the virulence of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Serratia marcescens<\/jats:italic>\n                    <\/jats:styled-content>\n                    PXG6 against\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    and identified two putative virulence factors,\n                    <jats:italic>ser<\/jats:italic>\n                    (serralysin) and\n                    <jats:italic>shlB<\/jats:italic>\n                    (hemolysin transporter). Whole\u2010genome sequencing revealed a GC content of 59.65%, 4650 predicted genes, multiple secretion systems (types I, V, and VI), and 715 putative virulence\u2010related genes. Bioinformatic analyses predicted type IV pili, serralysin, hemolysin, and flagella as pathogenic determinants. Purified prodigiosin exhibited concentration\u2010dependent insecticidal activity, whereas bacterial proteins alone caused little larval mortality, indicating that full virulence depends on live bacterial cells. Gene knockout mutants \u0394\n                    <jats:italic>ser<\/jats:italic>\n                    and \u0394\n                    <jats:italic>shlB<\/jats:italic>\n                    exhibited altered growth, motility, biofilm formation, and stress tolerance. Deletion of\n                    <jats:italic>shlB<\/jats:italic>\n                    significantly reduced hemolytic activity, protein secretion, and virulence against\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    , whereas \u0394\n                    <jats:italic>ser<\/jats:italic>\n                    had minimal impact on larval survival. Overall, PXG6 pathogenicity involves multiple bacterial components, with\n                    <jats:italic>shlB<\/jats:italic>\n                    playing a key role in virulence. These findings improve our understanding of the pathogenic mechanisms of PXG6 and support the development of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. marcescens<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010based biocontrol strategies against\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70391","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T01:34:38Z","timestamp":1786930478000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome\u2010Guided Dissection of\n                    <i>ser<\/i>\n                    and\n                    <i>shlB<\/i>\n                    Uncovers Multifactorial Virulence Mechanisms of\n                    <i>Serratia marcescens<\/i>\n                    <scp>PXG6<\/scp>\n                    in\n                    <i>Plutella xylostella<\/i>"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9647-1530","authenticated-orcid":false,"given":"Muhammad Rehan","family":"Akhtar","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity College of Plant Protection, Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Key Laboratory of Integrated Pest Management for Fujian\u2010Taiwan Crops Ministry of Agriculture and Rural Affairs of the People's Republic of China, Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Joint International Research Laboratory of Ecological Pest Control Ministry of\u00a0Education  Fuzhou China"},{"name":"Youxi\u2010Yangzhong Vegetable Pest Prevention and Control Fujian Observation and Research Station  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1358-8382","authenticated-orcid":false,"given":"Jun","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity College of Plant Protection, Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Key Laboratory of Integrated Pest Management for Fujian\u2010Taiwan Crops Ministry of Agriculture and Rural Affairs of the People's Republic of China, Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Joint International Research Laboratory of Ecological Pest Control Ministry of\u00a0Education  Fuzhou China"},{"name":"Youxi\u2010Yangzhong Vegetable Pest Prevention and Control Fujian Observation and Research Station  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5450-9687","authenticated-orcid":false,"given":"Yan","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity College of Plant Protection, Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Key Laboratory of Integrated Pest Management for Fujian\u2010Taiwan Crops Ministry of Agriculture and Rural Affairs of the People's Republic of China, Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Joint International Research Laboratory of Ecological Pest Control Ministry of\u00a0Education  Fuzhou China"},{"name":"Youxi\u2010Yangzhong Vegetable Pest Prevention and Control Fujian Observation and Research Station  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9522-7459","authenticated-orcid":false,"given":"Xiaofeng","family":"Xia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity College of Plant Protection, Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Key Laboratory of Integrated Pest Management for Fujian\u2010Taiwan Crops Ministry of Agriculture and Rural Affairs of the People's Republic of China, Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Joint International Research Laboratory of Ecological Pest Control Ministry of\u00a0Education  Fuzhou China"},{"name":"Youxi\u2010Yangzhong Vegetable Pest Prevention and Control Fujian Observation and Research Station  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0231625"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.71375\/djvs.2024.02102"},{"key":"e_1_2_11_4_1","volume-title":"The Effects of Insectary Plants on Natural Enemy and Pest Abundance, and Pest Dispersal Within Cabbage Agroecosystems in Hawai\u02bbi","author":"Armstrong K.","year":"2023"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-443-28849-4.00118-1"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2023.127422"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki008"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.3.833-836.2000"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03267-24"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29071466"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.01.003"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.35193\/bseufbd.1172976"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-026-04782-6"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11676-020-01122-w"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-014-0174-y"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-120811-153605"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2017.03.006"},{"key":"e_1_2_11_18_1","volume-title":"Secretion of the Chitinolytic Machinery in Serratia marcescens","author":"Hamilton J.","year":"2013"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.14715\/cmb\/2023.69.11.24"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.929150"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-119"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics15040359"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/fermentation9070637"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01582-06"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejbt.2019.04.007"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00661-10"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2022.104010"},{"key":"e_1_2_11_28_1","first-page":"3628","article-title":"Overwintering of the Diamondback Moth, Plutella xylostella in Temperate Countries","volume":"30","author":"Ma C.","year":"2010","journal-title":"Acta Ecologica Sinica"},{"key":"e_1_2_11_29_1","first-page":"2237","article-title":"Bacterial Bioagents for Insect Pest Management","volume":"5","author":"Mampallil L. J.","year":"2017","journal-title":"Journal of Entomology and Zoology Study"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-80644-3"},{"key":"e_1_2_11_31_1","article-title":"Enzyme Activity and Biodegradation Potentials of \n                     Serratia marcescens\n                   Cell\u2010Free Extract on Polyethylene Terephthalate Sheet","volume":"46","author":"Oluwadamilola E. O.","year":"2024","journal-title":"Applied Environmental Research"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-023-03633-6"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-011-2365-9"},{"key":"e_1_2_11_34_1","unstructured":"PHI\u2010base.2026.\u201cPathogen\u2010Host Interaction.\u201dhttp:\/\/www.phi\u2010base.org\/Phobius."},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.16943\/ptinsa\/2014\/v80i2\/3"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dsaf001"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.17582\/journal.jis\/2019\/5.2.89.94"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins9110364"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/09583150500136956"},{"key":"e_1_2_11_40_1","unstructured":"SecretomeP. n.d.https:\/\/services.healthtech.dtu.dk\/services\/SecretomeP\u20102.0\/."},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.47.091201.145309"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1211999"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.25083\/rbl\/25.1\/1275.1280"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti054"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2013-0864"},{"key":"e_1_2_11_46_1","unstructured":"TCDB. n.d.http:\/\/www.membranetransport.org\/transportDB2\/index.html."},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.agee.2025.109567"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.15112"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.105119"},{"key":"e_1_2_11_50_1","first-page":"D613","article-title":"PHI\u2010Base: The Pathogen\u2010Host Interactions Database","volume":"48","author":"Urban M.","year":"2020","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_11_51_1","unstructured":"VFDB.2026.\u201cVirulence Factors of Pathogenic Bacteria Core Database.\u201dhttps:\/\/www.mgc.ac.cn\/VFs\/."},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.793202"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1603\/EC12107"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1681911"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2022.111978"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2025.1672468"}],"container-title":["Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.70391","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1462-2920.70391","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1462-2920.70391","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T05:47:32Z","timestamp":1787636852000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1462-2920.70391"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":55,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/1462-2920.70391"],"URL":"https:\/\/doi.org\/10.1111\/1462-2920.70391","archive":["Portico"],"relation":{},"ISSN":["1462-2912","1462-2920"],"issn-type":[{"value":"1462-2912","type":"print"},{"value":"1462-2920","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70391"}}