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Here we present a genomic and metabolic study into the biogeochemistry of a uranium\u2010contaminated surficial aquifer site in Riverton, WY. We identified unique communities that varied based on geochemistry, geography, and compartment, matching other microbial subsurface studies. Cross\u2010site metabolism tests showed communities had functional capabilities of nitrogen respiration, manganese reduction, iron reduction, and sulfide oxidation. None of the sites displayed evidence of direct U\u2010bioreduction nor ammonium oxidation. Only former tailings area sites nearest a retention pond and a downgradient oxbow lake exhibited sulfate reduction metabolisms. This was contrary to our hypothesis of near\u2010river downgradient groundwater sites having U and S reduction capability. Most communities which showed S reduction capacity exhibited Fe oxidation capacity. Modelling demonstrated U as calcium uranyl carbonates. Based on our metabolism tests and known mineral and microbial metabolism reduction potentials, this suggests U reduction could be achieved via a secondary reaction with biogenically\u2010formed sulfide. Of 11 sites tested, this is possible in four. Microbial metabolisms were analysed for capacity to influence U reduction\u2013oxidation and provide microbial context to site remediation enhanced flushing efforts.<\/jats:p>","DOI":"10.1111\/1462-2920.70411","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T04:44:51Z","timestamp":1788929091000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Aquifer Microbial Communities Differentially Display Metabolisms Capable of Secondary Effects on Uranium Speciation Across a Former Metal Processing Site"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4510-9929","authenticated-orcid":false,"given":"Catherine J.","family":"Pettinger","sequence":"first","affiliation":[{"name":"Department of Bacteriology University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0091-6894","authenticated-orcid":false,"given":"Allondra M.","family":"Woods","sequence":"additional","affiliation":[{"name":"Department of Bacteriology University of Wisconsin\u2010Madison  Madison Wisconsin USA"},{"name":"Biological Engineering Department Utah State University  Logan Utah USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6112-438X","authenticated-orcid":false,"given":"Raymond H.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Contractor to the U.S. Department of Energy Office of Legacy Management RSI EnTech, LLC  Grand Junction Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1072-3988","authenticated-orcid":false,"given":"Charles J.","family":"Paradis","sequence":"additional","affiliation":[{"name":"Department of Geosciences University of Wisconsin at Milwaukee  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3444-3881","authenticated-orcid":false,"given":"Erica L.\u2010W.","family":"Majumder","sequence":"additional","affiliation":[{"name":"Department of Bacteriology University of Wisconsin\u2010Madison  Madison Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.1c04664"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13219"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781439833544"},{"key":"e_1_2_11_5_1","unstructured":"Applied Studies and Technology.2023.\u201cApplied Studies and Technology Persistent Secondary Contaminant Sources Data Release From Field Tracer Testing Studies at the Riverton Wyoming Processing Site; Doc. 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