{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:56:52Z","timestamp":1790913412490,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP\u20101516"],"award-info":[{"award-number":["PIP\u20101516"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015890","name":"Agencia Santafesina de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["DTT\u20102023\u2010062"],"award-info":[{"award-number":["DTT\u20102023\u2010062"]}],"id":[{"id":"10.13039\/501100015890","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020087","name":"Fundaci\u00f3n Williams","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020087","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Manganese oxidation in\n \n Pseudomonas resinovorans<\/jats:italic>\n <\/jats:styled-content>\n strain MOB\u2010513 has been previously linked to biofilm formation via the second messenger bis\u2010(3\u2032\u20135\u2032)\u2010cyclic dimeric GMP (c\u2010di\u2010GMP), although the specific molecular components mediating this regulation have not yet been identified. Here we investigate the genetic network underlying Mn(II) oxidation in MOB\u2010513. After transposon mutagenesis, several mutants with differences in Mn(II) oxidation were identified. Targeted analysis of the non\u2010oxidising mutants with Tn insertion disrupting\n pilC<\/jats:italic>\n ,\n algR<\/jats:italic>\n , and\n hk05390<\/jats:italic>\n genes, encoding a core Type IV pilus (Tfp) component, a regulator of pilus biogenesis, and an uncharacterised histidine kinase, respectively, revealed that their phenotype correlated with impaired biofilm formation, reduced intracellular c\u2010di\u2010GMP levels, and decreased Tfp production. We identified two regulators associated with a c\u2010di\u2010GMP\u2010dependent pathway linked to Mn(II) oxidation in MOB\u2010513. The hyper\u2010oxidising strain containing a transposon insertion in the promoter region of the\n dgc05792<\/jats:italic>\n gene showed upregulation of multiple Mn(II)\u2010oxidising enzymes. RR05389 further modulates intracellular c\u2010di\u2010GMP levels and Mn(II) oxidation and functions as a putative cognate response regulator with the histidine kinase HK05390. Together, these results expand our understanding of the molecular pathways underlying Mn(II) oxidation and identify new targets for optimising biological manganese removal.\n <\/jats:p>","DOI":"10.1111\/1462-2920.70423","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:37:06Z","timestamp":1790912226000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Molecular Pathways Underlying Mn(\n II<\/scp>\n ) Oxidation in\n \n Pseudomonas resinovorans<\/i>\n <\/scp>"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7966-3450","authenticated-orcid":false,"given":"Ainel\u00e9n","family":"Piazza","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology John Innes Centre Norwich UK"},{"name":"The Centre for Microbial Interactions Norwich UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Parra","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Universidad Nacional de Rosario Rosario Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2015-9814","authenticated-orcid":false,"given":"Marilina","family":"Gaffuri","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Universidad Nacional de Rosario Rosario Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1959-6820","authenticated-orcid":false,"given":"Jacob G.","family":"Malone","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology John Innes Centre Norwich UK"},{"name":"The Centre for Microbial Interactions Norwich UK"},{"name":"School of Biological Sciences University of East Anglia Norwich UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6039-7837","authenticated-orcid":false,"given":"Jorgelina","family":"Ottado","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Universidad Nacional de Rosario Rosario Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8730-8248","authenticated-orcid":false,"given":"Natalia","family":"Gottig","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Universidad Nacional de Rosario Rosario Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/978-94-007-7500-8_7","article-title":"Manganese in Health and Disease","volume":"13","author":"Avila D. 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