{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:50:50Z","timestamp":1791337850288,"version":"4.3.1"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000270","name":"Natural Environment Research Council","doi-asserted-by":"publisher","award":["NE\/X00547X\/1"],"award-info":[{"award-number":["NE\/X00547X\/1"]}],"id":[{"id":"10.13039\/501100000270","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000270","name":"Natural Environment Research Council","doi-asserted-by":"publisher","award":["NE\/X005259\/1"],"award-info":[{"award-number":["NE\/X005259\/1"]}],"id":[{"id":"10.13039\/501100000270","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["219551\/Z\/19\/Z"],"award-info":[{"award-number":["219551\/Z\/19\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/X020258\/1"],"award-info":[{"award-number":["MR\/X020258\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Monitoring fungal bioaerosols is essential for understanding their impacts on human health and crop productivity. However, current monitoring approaches rely on culture\u2010dependent techniques, which underestimate the diversity of fungal exposures. Adhesive\u2010coated films can effectively capture airborne particles but have been regarded as unsuitable for downstream DNA analysis, owing to the lack of standardised DNA extraction methods for the adhesive capture surface. Here, we present an optimised DNA extraction protocol for adhesive film, which we developed and validated to enable direct and culture\u2010independent ITS2\u2010based metabarcoding of fungal bioaerosols collected on a 96\u2010well plate sealing film (\u2018Sticky sampler\u2019). This validated approach was applied to field samples collected from four agricultural farms and from a domestic garden to evaluate seasonal and spatial changes in fungal bioaerosols. Our analysis uncovered pronounced site\u2010specific contrasts linked to geography and land management, as well as clear seasonal shifts in garden fungal communities. This study demonstrates that adhesive samplers, combined with optimised DNA extraction, provide a practical and inexpensive tool for fungal bioaerosol research by resolving ecologically meaningful spatiotemporal dynamics of airborne fungi. This protocol opens new opportunities for highly scalable surveillance of environmental bioaerosols.<\/jats:p>","DOI":"10.1111\/1462-2920.70431","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:10:43Z","timestamp":1791335443000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Direct\n                    <scp>DNA<\/scp>\n                    Extraction Workflow for Metabarcoding Fungal Bioaerosols From Adhesive Samplers"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-3011-9108","authenticated-orcid":false,"given":"Jiwon","family":"Jung","sequence":"first","affiliation":[{"name":"Department of Infectious Disease Epidemiology School of Public Health, Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9861-7281","authenticated-orcid":false,"given":"Rodrigo","family":"Leitao","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease Epidemiology School of Public Health, Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5680-4629","authenticated-orcid":false,"given":"Alice L. F.","family":"Day","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease Epidemiology School of Public Health, Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6123-5351","authenticated-orcid":false,"given":"Huw","family":"Davis","sequence":"additional","affiliation":[{"name":"Niab  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3389-0436","authenticated-orcid":false,"given":"Nichola","family":"Hawkins","sequence":"additional","affiliation":[{"name":"Niab  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6324-4123","authenticated-orcid":false,"given":"Kostya","family":"Kanyuka","sequence":"additional","affiliation":[{"name":"Niab  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1862-6402","authenticated-orcid":false,"given":"Matthew C.","family":"Fisher","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease Epidemiology School of Public Health, Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1111\/j.1469-8137.2009.03160.x","article-title":"The UNITE Database for Molecular Identification of Fungi \u2013 Recent Updates and Future Perspectives","volume":"186","author":"Abarenkov K.","year":"2010","journal-title":"New Phytologist"},{"key":"e_1_2_11_3_1","volume-title":"UNITE General FASTA Release for Fungi, Version 10.0","author":"Abarenkov K. 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G.","year":"2016","journal-title":"Microbial Risk Analysis"},{"key":"e_1_2_11_36_1","first-page":"101","volume-title":"Advances in Ecological Research","author":"Whitby C.","year":"2022"},{"key":"e_1_2_11_37_1","first-page":"315","volume-title":"PCR \u2010 Protocols and Applications \u2010 A Laboratory Manual","author":"White T. 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