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Herein, we queried the effect of\n                    <jats:italic>E. aedis<\/jats:italic>\n                    infection on the bacterial microbiota of adult female\n                    <jats:italic>A. aegypti<\/jats:italic>\n                    using high\u2010throughput amplicon sequencing of the 16S rRNA gene. Analysis of sequencing data revealed that the bacterial microbiota community is strikingly robust to\n                    <jats:italic>E. aedis<\/jats:italic>\n                    infection, as we observed no significant effect on alpha or beta diversity, differential abundance of any taxa, predicted metabolic function profile, or bacterial load. The data show that\n                    <jats:italic>E. aedis<\/jats:italic>\n                    , despite dramatically impacting the health and fitness of the adult female mosquito, does not affect the microbiota. These results provide unique insight into tripartite relationships (or lack thereof) between hosts, pathogens, and the microbiota.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70437","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:20:28Z","timestamp":1791354028000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Adult\n                    <i>Aedes aegypti<\/i>\n                    Bacterial Microbiota is Robust to Infection with the Obligate Microsporidian Parasite\n                    <i>Edhazardia aedis<\/i>"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1552-0810","authenticated-orcid":false,"given":"Dom","family":"Magistrado","sequence":"first","affiliation":[{"name":"Department of Entomology, College of Food, Agricultural, and Environmental Sciences The Ohio State University  Columbus Ohio USA"},{"name":"Department of Microbiology and Immunology University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1074-8982","authenticated-orcid":false,"given":"Sarah M.","family":"Short","sequence":"additional","affiliation":[{"name":"Department of Entomology, College of Food, Agricultural, and Environmental Sciences The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_2_12_2_1","volume-title":"16S Metagenomic Sequencing Library Preparation: Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System","year":"2013"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006586131235"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248\u2010022\u201002050\u20104"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/00949650215733"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep34084"},{"issue":"5","key":"e_1_2_12_7_1","first-page":"762","article-title":"Serratia odorifera\n                   Mediated Enhancement in Susceptibility of \n                     Aedes aegypti\n                   for Chikungunya Virus","volume":"139","author":"Apte\u2010Deshpande A. 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