{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T01:48:57Z","timestamp":1791856137272,"version":"4.3.4"},"reference-count":43,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32572914"],"award-info":[{"award-number":["32572914"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42221005"],"award-info":[{"award-number":["42221005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2024ZD49"],"award-info":[{"award-number":["ZR2024ZD49"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The reliance on conventional antibiotics and fungicides in food and agricultural systems drives drug\u2010resistant pathogen spread and environmental contamination. Lipopeptides represent an eco\u2010friendly alternative, but their efficacy is often limited by the presence of homologues with varying activities. Previously, we identified a novel lipopeptide, iturin W, whose C\n                    <jats:sub>14<\/jats:sub>\n                    variant exhibits superior efficacy against foodborne pathogens, whereas the C\n                    <jats:sub>15<\/jats:sub>\n                    homologue shows greater potency against plant pathogens. Notably, sorbitol supplementation shifts the homologue ratio from C\n                    <jats:sub>15<\/jats:sub>\n                    towards C\n                    <jats:sub>14<\/jats:sub>\n                    in iturin W. Here, we integrated transcriptomics, gene knockout and fermentation analyses to uncover the underlying mechanism. Our findings demonstrate that sorbitol induces metabolic reprogramming by enhancing glycolysis and oxidative phosphorylation while suppressing arginine and histidine biosynthesis. Key factors influencing this metabolic flux include SplA, DeoR\/GlpR, RpoN and ResE. Importantly, knockout of\n                    <jats:italic>splA<\/jats:italic>\n                    significantly boosts the yield of C\n                    <jats:sub>14<\/jats:sub>\n                    iturin W. Furthermore, high aeration combined with histidine\/arginine shifts production towards the C\n                    <jats:sub>15<\/jats:sub>\n                    variant, while low aeration with sorbitol enriches the C\n                    <jats:sub>14<\/jats:sub>\n                    form. Collectively, this work provides a mechanistic basis for rationally engineering microbial strains to optimize lipopeptide profiles, enabling precise biocontrol of bacterial versus fungal pathogens, thereby reducing reliance on synthetic chemicals and mitigating antimicrobial resistance in food and agricultural systems.\n                  <\/jats:p>","DOI":"10.1111\/1462-2920.70453","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T01:15:12Z","timestamp":1791854112000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sorbitol\u2010Mediated Shift of\n                    <scp>\n                      C\n                      <sub>14<\/sub>\n                    <\/scp>\n                    \/\n                    <scp>\n                      C\n                      <sub>15<\/sub>\n                    <\/scp>\n                    Iturin W Homologue Ratio in\n                    <i>Bacillus<\/i>\n                    <scp>sp. wsm\u20101<\/scp>"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1311-0638","authenticated-orcid":false,"given":"Jiao","family":"Chen","sequence":"first","affiliation":[{"name":"College of Life Sciences, Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1694-3114","authenticated-orcid":false,"given":"Yingyu","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4802-184X","authenticated-orcid":false,"given":"Chaomin","family":"Sun","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Experimental Marine Biology Institute of Oceanology, Chinese Academy of Sciences  Qingdao China"},{"name":"Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0168-8279","authenticated-orcid":false,"given":"Shimei","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201059872\u2010w"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/1983\u201021252024v3712462rc"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/b416648p"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0922\u2010338X(90)90155\u2010P"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03247\u201025"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods14020207"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1107\/s2053230x25003887"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2008.05.004"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.186.7.2028\u20102037.2004"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.792755"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12020107"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.183.24.7126\u20107134.2001"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c06329"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010021\u201001585\u20104"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010019\u20101089\u2010x"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2024.136725"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040\u20104039(00)87534\u20106"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1001\u20100742(09)60031\u20100"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25189949"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.64122\/YRKZ2709"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.26464"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.180.23.6154\u20106163.1998"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2025.111599"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2015.11.001"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11119\u2010016\u20109444\u2010y"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.28308"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0167\u20104781(97)00183\u20108"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00827\u201010"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2024.112730"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.03342"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274\u2010024\u201003908\u20100"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010024\u201002373\u20106"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203\u2010021\u201002401\u20108"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp120"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102958.1991.tb00829.x"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010012\u20103987\u20102"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010019\u20101139\u20104"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12163128"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102958.2000.02076.x"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201020548\u20108"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c03120"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01597\u201020"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.02744\u201017"}],"container-title":["Environmental 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