{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:56:19Z","timestamp":1791989779532,"version":"4.4.0"},"reference-count":46,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Microbiology and Infection"],"published-print":{"date-parts":[[2014,12]]},"DOI":"10.1111\/1469-0691.12710","type":"journal-article","created":{"date-parts":[[2014,6,9]],"date-time":"2014-06-09T08:14:41Z","timestamp":1402301681000},"page":"O1020-O1027","source":"Crossref","is-referenced-by-count":36,"title":["A multicenter prospective trial to asses a new real-time polymerase chain reaction for detection of Treponema pallidum, herpes simplex-1\/2 and Haemophilus ducreyi in genital, anal and oropharyngeal ulcers"],"prefix":"10.1016","volume":"20","author":[{"given":"M.","family":"Glatz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Juricevic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Altwegg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Bruisten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Komericki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Lautenschlager","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Weber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.P.","family":"Bosshard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1111\/1469-0691.12710_bib1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1097\/OLQ.0b013e3181bf5a98","article-title":"Etiology of genital ulcer disease. A prospective study of 278 cases seen in an STD clinic in Paris","volume":"37","author":"Hope\u2010Rapp","year":"2010","journal-title":"Sex Transm Dis"},{"key":"10.1111\/1469-0691.12710_bib2","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/978-1-61779-937-2_6","article-title":"The molecular diagnosis of sexually transmitted genital ulcer disease","volume":"903","author":"Chen","year":"2012","journal-title":"Methods Mol Biol"},{"key":"10.1111\/1469-0691.12710_bib3","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1128\/JCM.39.2.601-605.2001","article-title":"Diagnosing genital ulcer disease in a clinic for sexually transmitted diseases in Amsterdam, The Netherlands","volume":"39","author":"Bruisten","year":"2001","journal-title":"J Clin Microbiol"},{"key":"10.1111\/1469-0691.12710_bib4","doi-asserted-by":"crossref","first-page":"e44094","DOI":"10.1371\/journal.pone.0044094","article-title":"A systematic review and meta\u2010analysis of risk factors for sexual transmission of HIV in India","volume":"7","author":"Arora","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1111\/1469-0691.12710_bib5","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1111\/odi.12058","article-title":"Oral syphilis: a retrospective analysis of 12 cases and a review of the literature","volume":"19","author":"Leuci","year":"2012","journal-title":"Oral Dis"},{"key":"10.1111\/1469-0691.12710_bib6","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1097\/00007435-199901000-00009","article-title":"The etiology of genital ulcer disease by multiplex polymerase chain reaction and relationship to HIV infection among patients attending sexually transmitted disease clinics in Pune, India","volume":"26","author":"Risbud","year":"1999","journal-title":"Sex Transm Dis"},{"key":"10.1111\/1469-0691.12710_bib7","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1258\/ijsa.2008.008279","article-title":"UK national guidelines on the management of syphilis 2008","volume":"19","author":"Kingston","year":"2008","journal-title":"Int J STD AIDS"},{"key":"10.1111\/1469-0691.12710_bib8","first-page":"1","article-title":"Sexually transmitted diseases treatment guidelines, 2010","volume":"59","author":"Workowski","year":"2010","journal-title":"MMWR Recomm Rep"},{"key":"10.1111\/1469-0691.12710_bib9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1258\/ijsa.2010.010278","article-title":"European guideline for the management of genital herpes, 2010","volume":"22","author":"Patel","year":"2011","journal-title":"Int J STD AIDS"},{"key":"10.1111\/1469-0691.12710_bib10","doi-asserted-by":"crossref","DOI":"10.2807\/ese.15.44.19703-en","article-title":"Guidelines for the laboratory diagnosis of genital herpes in eastern European countries","volume":"15","author":"Domeika","year":"2010","journal-title":"Euro Surveill"},{"key":"10.1111\/1469-0691.12710_bib11","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1093\/cid\/cis757","article-title":"Serological response to treatment of syphilis according to disease stage and HIV status","volume":"55","author":"Knaute","year":"2012","journal-title":"Clin Infect Dis"},{"key":"10.1111\/1469-0691.12710_bib12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/CMR.8.1.1","article-title":"Laboratory diagnosis and interpretation of tests for syphilis","volume":"8","author":"Larsen","year":"1995","journal-title":"Clin Microbiol Rev"},{"key":"10.1111\/1469-0691.12710_bib13","first-page":"30","article-title":"Darkfield microscopy for point\u2010of\u2010care syphilis diagnosis","volume":"43","author":"Pierce","year":"2011","journal-title":"MLO Med Lab Obs"},{"key":"10.1111\/1469-0691.12710_bib14","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1136\/sti.78.3.160","article-title":"Using the evidence base on genital herpes: optimising the use of diagnostic tests and information provision","volume":"78","author":"Scoular","year":"2002","journal-title":"Sex Transm Infect"},{"key":"10.1111\/1469-0691.12710_bib15","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1155\/2005\/851610","article-title":"The laboratory diagnosis of Haemophilus ducreyi","volume":"16","author":"Alfa","year":"2005","journal-title":"Can J Infect Dis Med Microbiol"},{"key":"10.1111\/1469-0691.12710_bib16","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/978-1-61779-937-2_9","article-title":"Protocols for detection and typing of Treponema pallidum using PCR methods","volume":"903","author":"Bruisten","year":"2012","journal-title":"Methods Mol Biol"},{"key":"10.1111\/1469-0691.12710_bib17","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1128\/JCM.34.1.49-54.1996","article-title":"Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers","volume":"34","author":"Orle","year":"1996","journal-title":"J Clin Microbiol"},{"key":"10.1111\/1469-0691.12710_bib18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1373\/49.1.1","article-title":"Towards complete and accurate reporting of studies of diagnostic accuracy: the stard initiative. Standards for reporting of diagnostic accuracy","volume":"49","author":"Bossuyt","year":"2003","journal-title":"Clin Chem"},{"key":"10.1111\/1469-0691.12710_bib19","series-title":"Verification and validation of multiplex nucleid acid assays: approved guidelines. CLSI document MM17\u2010A","author":"Clinical and Laboratory Standards Institutes","year":"2008"},{"key":"10.1111\/1469-0691.12710_bib20","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1093\/bioinformatics\/btm091","article-title":"Enhancements and modifications of primer design program primer3","volume":"23","author":"Koressaar","year":"2007","journal-title":"Bioinformatics"},{"key":"10.1111\/1469-0691.12710_bib21","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1002\/jmv.20365","article-title":"Differentiation of herpes simplex virus types 1 and 2 in clinical samples by a real\u2010time taqman PCR assay","volume":"76","author":"Corey","year":"2005","journal-title":"J Med Virol"},{"key":"10.1111\/1469-0691.12710_bib22","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.mimet.2005.09.007","article-title":"The copy\u2010number of plasmids and other genetic elements can be determined by SYBR\u2010green\u2010based quantitative real\u2010time PCR","volume":"65","author":"Providenti","year":"2006","journal-title":"J Microbiol Methods"},{"key":"10.1111\/1469-0691.12710_bib23","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1128\/JCM.39.2.485-493.2001","article-title":"Purification and characterization of PCR\u2010inhibitory components in blood cells","volume":"39","author":"Al\u2010Soud","year":"2001","journal-title":"J Clin Microbiol"},{"key":"10.1111\/1469-0691.12710_bib24","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1186\/1471-2105-13-134","article-title":"Primer\u2010blast: a tool to design target\u2010specific primers for polymerase chain reaction","volume":"13","author":"Ye","year":"2012","journal-title":"BMC Bioinformatics"},{"key":"10.1111\/1469-0691.12710_bib25","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1111\/j.1469-0691.2006.01566.x","article-title":"Specific and sensitive diagnosis of syphilis using a real\u2010time PCR for Treponema pallidum","volume":"12","author":"Koek","year":"2006","journal-title":"Clin Microbiol Infect"},{"key":"10.1111\/1469-0691.12710_bib26","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1128\/JCM.41.2.576-580.2003","article-title":"Diagnosing herpesvirus infections by real\u2010time amplification and rapid culture","volume":"41","author":"van Doornum","year":"2003","journal-title":"J Clin Microbiol"},{"key":"10.1111\/1469-0691.12710_bib27","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1128\/JCM.38.2.795-799.2000","article-title":"Diagnosis of herpes simplex virus infections in the clinical laboratory by lightcycler PCR","volume":"38","author":"Espy","year":"2000","journal-title":"J Clin Microbiol"},{"issue":"suppl 4","key":"10.1111\/1469-0691.12710_bib28","first-page":"iv19","article-title":"Chancroid","volume":"82","author":"Lewis","year":"2006","journal-title":"Sex Transm Infect"},{"key":"10.1111\/1469-0691.12710_bib29","author":"Core"},{"key":"10.1111\/1469-0691.12710_bib30","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jinf.2013.03.011","article-title":"Usefulness of IgM\u2010specific enzyme immunoassays for serodiagnosis of syphilis: comparative evaluation of three different assays","volume":"67","author":"Bosshard","year":"2013","journal-title":"J Infect"},{"key":"10.1111\/1469-0691.12710_bib31","first-page":"373","article-title":"Oral manifestations of sexually transmitted infections","volume":"147","author":"Mastrolorenzo","year":"2012","journal-title":"G Ital Dermatol Venereol"},{"key":"10.1111\/1469-0691.12710_bib32","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1128\/JCM.01578-06","article-title":"Development of a real\u2010time PCR assay to detect Treponema pallidum in clinical specimens and assessment of the assay's performance by comparison with serological testing","volume":"45","author":"Leslie","year":"2007","journal-title":"J Clin Microbiol"},{"key":"10.1111\/1469-0691.12710_bib33","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1136\/sti.2009.040451","article-title":"A new multiplex real\u2010time PCR test for HSV1\/2 and syphilis: an evaluation of its impact in the laboratory and clinical setting","volume":"86","author":"Scott","year":"2010","journal-title":"Sex Transm Infect"},{"key":"10.1111\/1469-0691.12710_bib34","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1136\/sti.2008.034207","article-title":"Evaluation of multiplex real\u2010time PCR for detection of Haemophilus ducreyi, Treponema pallidum, herpes simplex virus type 1 and 2 in the diagnosis of genital ulcer disease in the Rakai district, Uganda","volume":"85","author":"Suntoke","year":"2009","journal-title":"Sex Transm Infect"},{"key":"10.1111\/1469-0691.12710_bib35","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1086\/503424","article-title":"Detection and discrimination of herpes simplex viruses, Haemophilus ducreyi, Treponema pallidum, and Calymmatobacterium (Klebsiella) granulomatis from genital ulcers","volume":"42","author":"Mackay","year":"2006","journal-title":"Clin Infect Dis"},{"key":"10.1111\/1469-0691.12710_bib36","doi-asserted-by":"crossref","first-page":"355","DOI":"10.2340\/00015555-0241","article-title":"Herpes simplex virus infection and genital ulcer disease among patients with sexually transmitted infections in Dar es Salaam, Tanzania","volume":"87","author":"Nilsen","year":"2007","journal-title":"Acta Derm Venereol"},{"key":"10.1111\/1469-0691.12710_bib37","doi-asserted-by":"crossref","first-page":"e63953","DOI":"10.1371\/journal.pone.0063953","article-title":"Etiology of genital ulcer disease in a sexually transmitted infection reference center in Manaus, Brazilian Amazon","volume":"8","author":"Gomes Naveca","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1111\/1469-0691.12710_bib38","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1086\/315005","article-title":"Chancroid, primary syphilis, genital herpes, and lymphogranuloma venereum in Antananarivo, Madagascar","volume":"180","author":"Behets","year":"1999","journal-title":"J Infect Dis"},{"key":"10.1111\/1469-0691.12710_bib39","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.4269\/ajtmh.13-0315","article-title":"Simultaneous detection of seven sexually transmitted agents in human immunodeficiency virus\u2010infected Brazilian women by multiplex polymerase chain reaction","volume":"89","author":"Souza","year":"2013","journal-title":"Am J Trop Med Hyg"},{"key":"10.1111\/1469-0691.12710_bib40","first-page":"25","author":"European Centre for Disease Prevention and Control","year":"2011","journal-title":"Sexually transmitted infections in Europe 2011"},{"key":"10.1111\/1469-0691.12710_bib41","author":"Swiss Federal Office of Public Health"},{"key":"10.1111\/1469-0691.12710_bib42","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1097\/OLQ.0000000000000101","article-title":"Epidemiology of genital herpes simplex virus type 1 and 2 infections in southwestern Finland during a 10\u2010year period (2003\u20132012)","volume":"41","author":"Kortekangas\u2010Savolainen","year":"2014","journal-title":"Sex Transm Dis"},{"key":"10.1111\/1469-0691.12710_bib43","first-page":"818","article-title":"Eradicating chancroid","volume":"79","author":"Steen","year":"2001","journal-title":"Bull World Health Organ"},{"key":"10.1111\/1469-0691.12710_bib44","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1136\/sti.79.3.202","article-title":"Increasing relative prevalence of HSV\u20102 infection among men with genital ulcers from a mining community in South Africa","volume":"79","author":"Lai","year":"2003","journal-title":"Sex Transm Infect"},{"key":"10.1111\/1469-0691.12710_bib45","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1111\/j.1440-0960.2008.00439.x","article-title":"Cutaneous chancroid in a visitor from Vanuatu","volume":"49","author":"McBride","year":"2008","journal-title":"Australas J Dermatol"},{"key":"10.1111\/1469-0691.12710_bib46","doi-asserted-by":"crossref","first-page":"348","DOI":"10.5694\/j.1326-5377.2010.tb03537.x","article-title":"Chronic cutaneous ulcers secondary to Haemophilus ducreyi infection","volume":"192","author":"Peel","year":"2010","journal-title":"Med J Aust"}],"container-title":["Clinical Microbiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1198743X15600544?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1198743X15600544?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T15:42:27Z","timestamp":1597938147000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1198743X15600544"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12]]},"references-count":46,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["S1198743X15600544"],"URL":"https:\/\/doi.org\/10.1111\/1469-0691.12710","relation":{},"ISSN":["1198-743X"],"issn-type":[{"value":"1198-743X","type":"print"}],"subject":[],"published":{"date-parts":[[2014,12]]}}}