{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T21:39:46Z","timestamp":1787693986586,"version":"build-2784847793"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,11,27]],"date-time":"2012-11-27T00:00:00Z","timestamp":1353974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJOG"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the feasibility and utility of intraoperative post\u2010conisation human papillomavirus (<jats:styled-content style=\"fixed-case\">IOP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>) testing and cytology to detect treatment failure in patients with cervical intraepithelial neoplasia grades\u00a02\u20133 (<jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2\u20133).<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Prospective observational pilot study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>arcelona, <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>pain.<\/jats:p><\/jats:sec><jats:sec><jats:title>Population<\/jats:title><jats:p>A cohort of 132 women treated for <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2\u20133 by loop electrosurgical conisation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An endocervical sample was obtained intraoperatively with a cytobrush from the cervix remaining after the conisation. The material was kept in <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>reserv<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>yt medium and processed for <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>ybrid <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>apture\u00a02 and cytology. Patients were followed\u2010up for 24\u00a0months. The performance of <jats:styled-content style=\"fixed-case\">IOP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> testing and <jats:styled-content style=\"fixed-case\">IOP<\/jats:styled-content> cytology was compared with conventional indicators of recurrence (cone margin, endocervical curettage, and <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> testing and cytology at 6\u00a0months).<\/jats:p><\/jats:sec><jats:sec><jats:title>Main outcome measure<\/jats:title><jats:p>Treatment failure (i.e. recurrent <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2\u20133 during follow\u2010up).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Treatment failure was identified in 12 women (9.1%). <jats:styled-content style=\"fixed-case\">IOP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> testing for sensitivity, specificity, and positive and negative predictive values for treatment failure were 91.7, 78.3, 62.2, and 96.0%, respectively, which are similar to the figures for conventional <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> testing at 6\u00a0months (91.7, 76.0, 64.0, and 95.1%, respectively), and are better than the values of other conventional predictive factors (cone margin, endocervical curettage, and cytology intraoperative at 6\u00a0months). <jats:styled-content style=\"fixed-case\">IOP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> was strongly associated with treatment failure in the multivariate analysis (<jats:styled-content style=\"fixed-case\">OR<\/jats:styled-content> 15.40, 95%\u00a0<jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content> 1.58\u2013150.42).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">IOP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> testing is feasible, and accurately predicts treatment failure in patients with <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2\u20133. This new approach may allow an early identification of patients with treatment failure, thereby facilitating the scheduling of an attenuated follow\u2010up for negative patients who are at very low risk of persistent disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1471-0528.12072","type":"journal-article","created":{"date-parts":[[2012,11,29]],"date-time":"2012-11-29T00:03:53Z","timestamp":1354147433000},"page":"392-399","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Intraoperative post\u2010conisation human papillomavirus testing for early detection of treatment failure in patients with cervical intraepithelial neoplasia: a pilot study"],"prefix":"10.1111","volume":"120","author":[{"given":"A","family":"Torn\u00e9","sequence":"first","affiliation":[{"name":"Institut Clinic of Gynaecology, Obstetrics and Neonatology Hospital Cl\u00ednic\u2010Institut d\u2032Investigacions Biom\u00e8diques August Pi i Sunyer 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