{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T10:09:50Z","timestamp":1777370990976,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2016,2,5]],"date-time":"2016-02-05T00:00:00Z","timestamp":1454630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Guangdong Provincial Natural Science Foundation of China","award":["20120318067"],"award-info":[{"award-number":["20120318067"]}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJOG"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the expression of circular <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) in blood corpuscles of pregnant women before 20\u00a0weeks of pregnancy and to create a new model to identify the performance of circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> combined with protein factors for the early diagnosis of pre\u2010eclampsia (<jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Nested case\u2013control study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting<\/jats:title><jats:p>University medical centre, Guangzhou, China.<\/jats:p><\/jats:sec><jats:sec><jats:title>Population<\/jats:title><jats:p>A total of 1400 pregnant women recruited between 8 and 20\u00a0weeks of gestation. In all, 41 women with <jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content> were included in the study, and were matched with 41 normally pregnant women based on maternal age and gestational age at same\u2010size ratio.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The samples were analysed using a human circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> microarray in the discovery phase, then the circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and the plasma protein factor endoglin (<jats:styled-content style=\"fixed-case\">ENG<\/jats:styled-content>) were validated. Finally we combined <jats:styled-content style=\"fixed-case\">ENG<\/jats:styled-content> with circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> to create a new early prediction model for <jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Main outcome measures<\/jats:title><jats:p>Early changes of circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ENG<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The circ_101222 levels in blood corpuscles of patients with <jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content> were significantly higher than those in corresponding healthy women (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001). Using <jats:styled-content style=\"fixed-case\">ENG<\/jats:styled-content> in combination with circ_101222 resulted in a sensitivity of 0.7073, a specificity of 0.8049, and overall area under the curve of 0.876 (95% confidence interval 0.816\u20130.922) for the prediction of <jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and plasma proteins may have some predictive value for <jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content> (such as circ_101222 and <jats:styled-content style=\"fixed-case\">ENG<\/jats:styled-content>). The performance of each of these factors may be strengthened when plasma proteins are used in combination with circ<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>. The results are preliminary and need to be validated in larger studies and other populations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Tweetable abstract<\/jats:title><jats:p>Plasma protein endoglin in combination with circ_101222 strengthened the predictive power for pre\u2010eclampsia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1471-0528.13897","type":"journal-article","created":{"date-parts":[[2016,2,5]],"date-time":"2016-02-05T06:52:28Z","timestamp":1454655148000},"page":"2113-2118","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":94,"title":["Circular <scp>RNA<\/scp> in blood corpuscles combined with plasma protein factor for early prediction of pre\u2010eclampsia"],"prefix":"10.1111","volume":"123","author":[{"given":"Y\u2010G","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Guang Zhou Women and Children's Medical Centre of Guang Zhou Medical University Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u2010L","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Guang Zhou Women and Children's Medical Centre of Guang Zhou Medical University Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Guang Zhou Women and Children's Medical Centre of Guang Zhou Medical University Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W\u2010L","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Guang Zhou Women and Children's Medical Centre of Guang Zhou Medical University Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,2,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.13516"},{"key":"e_1_2_6_3_1","first-page":"519","article-title":"Colmenares MC [The prognosis for children of mothers with preeclampsia. 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