{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T12:01:04Z","timestamp":1780056064236,"version":"3.54.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,10,3]],"date-time":"2016-10-03T00:00:00Z","timestamp":1475452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"School of Laboratory Medicine and Medical Sciences, UKZN"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJOG"],"published-print":{"date-parts":[[2017,5]]},"abstract":"Objective<\/jats:title>To compare expression of markers of HIV<\/jats:styled-content> and associated receptors (p24, CD<\/jats:styled-content>4, CCR<\/jats:styled-content>5 and ICAM<\/jats:styled-content>\u20102) in placentae and umbilical cords of HIV<\/jats:styled-content>\u2010associated and pre\u2010eclamptic pregnancies to elucidate any association between these conditions in mother\u2010to\u2010child transmission.<\/jats:p><\/jats:sec>Design<\/jats:title>Cross\u2010sectional immunohistochemical analysis of target receptor expression.<\/jats:p><\/jats:sec>Setting<\/jats:title>Laboratory\u2010based study of primigravidae attending a district hospital in South Africa.<\/jats:p><\/jats:sec>Population or sample<\/jats:title>Retrospectively collected placental tissue (stratified into four groups according to HIV<\/jats:styled-content> status of normotensive and pre\u2010eclamptic participants (n<\/jats:italic>\u00a0=\u00a020\/group).<\/jats:p><\/jats:sec>Method<\/jats:title>Immunohistochemistry utilising CD<\/jats:styled-content>4 (1:1), p24 (1:10), CCR<\/jats:styled-content>5 (1:80) and ICAM<\/jats:styled-content>\u20102 (1:100) antibodies was performed using light microscopy for image acquisition and analysis.<\/jats:p><\/jats:sec>Main outcome measures<\/jats:title>Evaluate the expression of receptors on syncytiotrophoblast involved in in utero<\/jats:italic> transmission of HIV<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>Syncytiotrophoblast was immunopositive for CD<\/jats:styled-content>4 and CCR<\/jats:styled-content>5 antibody with greater expression of CCR<\/jats:styled-content>5 in HIV<\/jats:styled-content>\u2010positive versus HIV<\/jats:styled-content>\u2010negative groups (F<\/jats:italic>1,159<\/jats:sub>\u00a0=\u00a06.979, P\u00a0<\/jats:italic>=\u00a00.009) and normotensive versus pre\u2010eclamptic groups (F<\/jats:italic>1,159<\/jats:sub>\u00a0=\u00a08.803, P\u00a0<\/jats:italic>=\u00a00.003). p24 was present in both placentae and umbilical cords of babies that were HIV<\/jats:styled-content>\u2010negative at 6\u00a0weeks. ICAM<\/jats:styled-content>\u20102 immunostaining was observed in the syncytiotrophoblast across study groups and was significantly higher in the HIV<\/jats:styled-content>\u2010negative pre\u2010eclamptic group (\u03c7<\/jats:italic>2<\/jats:sup> (3) \u00a0=\u00a045.3; P<\/jats:italic>\u00a0<\u00a00.001).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Concurrent CD<\/jats:styled-content>4 and CCR<\/jats:styled-content>5 receptor expression demonstrates possible in utero<\/jats:italic> viral entry routes across the placental barrier. ICAM<\/jats:styled-content>\u20102 expression may influence HIV<\/jats:styled-content> passage across the placenta or restoration of risk of pre\u2010eclampsia in HAART<\/jats:styled-content>\u2010treated mothers. HIV<\/jats:styled-content> was found in fetal circulation regardless of antiretroviral treatment. Further confirmatory ultrastructural and molecular work is warranted.<\/jats:p><\/jats:sec>Tweetable abstract<\/jats:title>CD<\/jats:styled-content>4, CCR<\/jats:styled-content>5 and ICAM<\/jats:styled-content>\u20102 on syncytiotrophoblast may facilitate HIV<\/jats:styled-content> infection of passage across the placenta.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1471-0528.14311","type":"journal-article","created":{"date-parts":[[2016,10,11]],"date-time":"2016-10-11T16:17:58Z","timestamp":1476202678000},"page":"920-928","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The role of trophoblast cell receptor expression in HIV<\/scp>\u20101 passage across the placenta in pre\u2010eclampsia: an observational study"],"prefix":"10.1111","volume":"124","author":[{"given":"V","family":"Dorsamy","sequence":"first","affiliation":[{"name":"School of Laboratory Medicine and Medical Sciences College of Health Sciences University of 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