{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:41:09Z","timestamp":1790077269866,"version":"4.0.1"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:00:00Z","timestamp":1540339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJOG"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:sec><jats:title>\n<jats:bold>Objective<\/jats:bold>\n<\/jats:title><jats:p>Evaluating sexual function and quality of life (QoL) in patients treated with a modified Abb\u00e9\u2013McIndoe technique using in\u00a0vitro cultured autologous vaginal mucosa.<\/jats:p><\/jats:sec><jats:sec><jats:title>\n<jats:bold>Design<\/jats:bold>\n<\/jats:title><jats:p>Descriptive study.<\/jats:p><\/jats:sec><jats:sec><jats:title>\n<jats:bold>Setting<\/jats:bold>\n<\/jats:title><jats:p>Policlinico Umberto I, Sapienza University of Rome.<\/jats:p><\/jats:sec><jats:sec><jats:title>\n<jats:bold>Population<\/jats:bold>\n<\/jats:title><jats:p>From 2006 to 2016, 39 women affected by Mayer\u2013Rokitansky\u2013K\u00fcster\u2013Hauser syndrome (<jats:styled-content style=\"fixed-case\">MRKHS<\/jats:styled-content>) underwent vaginoplasty at our centre using a modified Abb\u00e9\u2013McIndoe technique with in\u00a0vitro cultured autologous vaginal tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>\n<jats:bold>Methods<\/jats:bold>\n<\/jats:title><jats:p>For each patient, vaginal tissue was obtained by full\u2010thickness biopsy of the vaginal vestibule. Following enzymatic dissociation, cells were cultured for 2\u20133\u00a0weeks before the transplant.<\/jats:p><\/jats:sec><jats:sec><jats:title>\n<jats:bold>Main outcome measures<\/jats:bold>\n<\/jats:title><jats:p>Each patient completed two validated questionnaires to quantify sexual function and QoL: the Female Sexual Function Index (<jats:styled-content style=\"fixed-case\">FSFI<\/jats:styled-content>), administered at 12, 36, and 60\u00a0months, and the Psychological General Well Being Index (<jats:styled-content style=\"fixed-case\">PGWBI<\/jats:styled-content>) administered at 0, 6, and 36\u00a0months after surgery.<\/jats:p><\/jats:sec><jats:sec><jats:title>\n<jats:bold>Results<\/jats:bold>\n<\/jats:title><jats:p>Twelve months after surgery, 29 patients were engaging in regular sexual activity. The <jats:styled-content style=\"fixed-case\">FSFI<\/jats:styled-content> test results showed a satisfactory sexual function compared to the general population, with median values of 25.85 (range 4.6\u201330.5) at 12 months, 27.2 (range 4.4\u201333.6) at 36 months, and 29.6 (range 23.9\u201333.6) at 60 months. The <jats:styled-content style=\"fixed-case\">PGWBI<\/jats:styled-content> questionnaire showed a median score of 420.5 (range 108\u2013540) before surgery, and 459 (range 252\u2013533) at the 60\u2010month follow\u2010up.<\/jats:p><\/jats:sec><jats:sec><jats:title>\n<jats:bold>Conclusions<\/jats:bold>\n<\/jats:title><jats:p>Vaginoplasty performed with the use of autologous vaginal tissue, besides ensuring a long\u2010term satisfying sex life, helps in achieving an improvement in QoL that is maintained over time.<\/jats:p><\/jats:sec><jats:sec><jats:title>Tweetable abstract<\/jats:title><jats:p>Vaginoplasty using in\u00a0vitro vaginal tissue ensures a satisfactory sexual function and improves quality of life.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1471-0528.15477","type":"journal-article","created":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T19:25:56Z","timestamp":1537385156000},"page":"123-127","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Treatment of the Mayer\u2013Rokitansky\u2013K\u00fcster\u2013Hauser syndrome with autologous in\u00a0vitro cultured vaginal tissue: descriptive study of long\u2010term results and patient outcomes"],"prefix":"10.1111","volume":"126","author":[{"given":"I","family":"Sabatucci","sequence":"first","affiliation":[{"name":"Department of Gynecologica and Obstetrical Sciences and Urological Sciences Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Palaia","sequence":"additional","affiliation":[{"name":"Department of Gynecologica and Obstetrical Sciences and Urological Sciences Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Marchese","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Muzii","sequence":"additional","affiliation":[{"name":"Department of Gynecologica and Obstetrical Sciences and Urological Sciences Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C Della","family":"Morte","sequence":"additional","affiliation":[{"name":"Department of Gynecologica and Obstetrical Sciences and Urological Sciences Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Giorgini","sequence":"additional","affiliation":[{"name":"Department of Gynecologica and Obstetrical Sciences and Urological Sciences Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Musella","sequence":"additional","affiliation":[{"name":"Department of Gynecologica and Obstetrical Sciences and Urological Sciences Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Ceccarelli","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Vescarelli","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PB","family":"Panici","sequence":"additional","affiliation":[{"name":"Department of Gynecologica and Obstetrical Sciences and Urological Sciences Sapienza University of Rome Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006250-200207000-00034"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-5751-5-4"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-2-13"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/302452"},{"key":"e_1_2_6_6_1","first-page":"386","article-title":"Atypical form of Mayer\u2010Rokitansky\u2010K\u00fcster\u2010Hauser syndrome","volume":"37","author":"Schmid\u2010Tannwald I","year":"1977","journal-title":"Geburtshilfe Frauenheilkd"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(79)80514-4"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dem096"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(81)90029-6"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a019019"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmig.2014.09.012"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/009262300278597"},{"key":"e_1_2_6_13_1","unstructured":"DupuyHJ.The psychological general well\u2010being index. 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