{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:43:38Z","timestamp":1792172618964,"version":"4.4.0"},"reference-count":33,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2021,6,20]],"date-time":"2021-06-20T00:00:00Z","timestamp":1624147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJOG"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the impact of vaginal microbiota on pregnancy outcomes in women undergoing assisted reproduction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>A prospective cohort study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Setting<\/jats:title>\n                    <jats:p>A university\u2010based assisted reproductive technology (ART) centre.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Population<\/jats:title>\n                    <jats:p>223 women undergoing ART treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Prior to embryo transfer, vaginal samples were collected from the posterior fornix. Vaginal microbiota identification was carried out using next\u2010generation sequencing and categorised according to the V3\u2013V4 hypervariable region in the 16S rRNA gene region.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main outcome measures<\/jats:title>\n                    <jats:p>ART clinical outcomes (implantation, clinical pregnancy rates and live birth rates).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The live birth rate in women with community state type (CST)\u2010I (39%) was higher than that in women with CST\u2010III (21.5%) but the difference was not statistically significant (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.052). The relative abundance of\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      was lower in women who failed to become pregnant (NP group) (67.71%) than in women who became pregnant (PR group) (79.72%). However, this difference was not statistically significant (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.06). In the NP group, the relative abundance of\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      (7.81%) and\n                      <jats:italic>Gardnerella<\/jats:italic>\n                      (9.40%) was higher than that in the PR group (relative abundance of\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      and\n                      <jats:italic>Gardnerella<\/jats:italic>\n                      was 2.28% and 5.56%, respectively). The abundance of\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      was found to be statistically significantly different between the two study groups (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.014). Linear discriminant analysis (LDA) further validated that\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      had the highest contribution (LDA score\u2009&gt;4.0) to the difference between these two groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      has the highest contribution to the distinction between the PR and NP groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Tweetable abstract<\/jats:title>\n                    <jats:p>\n                      A relatively high abundance of\n                      <jats:italic>Streptococcus<\/jats:italic>\n                      in the vaginal microbiota may be associated with a lower ART success rate.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1471-0528.16782","type":"journal-article","created":{"date-parts":[[2021,5,30]],"date-time":"2021-05-30T06:16:50Z","timestamp":1622355410000},"page":"2101-2109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["The vaginal microbiota composition of women undergoing assisted reproduction: a prospective cohort study"],"prefix":"10.1111","volume":"128","author":[{"given":"A","family":"Karaer","sequence":"first","affiliation":[{"name":"Reproductive Science &amp; Advanced Bioinformatics Application &amp; Research Centre Inonu University Malatya Turkey"},{"name":"Division of Reproductive Endocrinology and Infertility Department of Obstetrics and Gynaecology School of Medicine Inonu University Malatya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Do\u011fan","sequence":"additional","affiliation":[{"name":"Reproductive Science &amp; Advanced Bioinformatics Application &amp; Research Centre Inonu University Malatya Turkey"},{"name":"Department of Biomedical Engineering School of Engineering Inonu University Malatya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"G\u00fcnal","sequence":"additional","affiliation":[{"name":"Reproductive Science &amp; Advanced Bioinformatics Application &amp; Research Centre Inonu University Malatya Turkey"},{"name":"Department of Pharmaceutical Microbiology School of Pharmacy Inonu University Malatya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Tuncay","sequence":"additional","affiliation":[{"name":"Reproductive Science &amp; Advanced Bioinformatics Application &amp; Research Centre Inonu University Malatya Turkey"},{"name":"Division of Reproductive Endocrinology and Infertility Department of Obstetrics and Gynaecology School of Medicine Inonu University Malatya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Arda D\u00fcz","sequence":"additional","affiliation":[{"name":"Reproductive Science &amp; Advanced Bioinformatics Application &amp; Research Centre Inonu University Malatya Turkey"},{"name":"Division of Reproductive Endocrinology and Infertility Department of Obstetrics and Gynaecology School of Medicine Inonu University Malatya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"\u00dcnver","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology School of Pharmacy Inonu University Malatya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Tecellio\u011flu","sequence":"additional","affiliation":[{"name":"Reproductive Science &amp; Advanced Bioinformatics Application &amp; Research Centre Inonu University Malatya Turkey"},{"name":"Division of Reproductive Endocrinology and Infertility Department of Obstetrics and Gynaecology School of Medicine Inonu University Malatya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)09417-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2015.17296"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68117-8"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dew026"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiy744"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ht9020012"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw099"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-6-r60"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002611107"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2017.04.005"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-019-0450-2"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-013-3064-9"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-116"},{"key":"e_1_2_7_15_1","first-page":"421","article-title":"Lactobacillus crispatus as biomarker of the healthy vaginal tract","volume":"74","author":"Lepargneur JP","year":"2016","journal-title":"Ann Biol Clin"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dez065"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofz525"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2016.11.007"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.15178"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02451"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00901-0"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2018.10.014"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.01.013"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1479-828X.2005.00490.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2018.06.036"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2018.00078"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu067"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2007.01.006"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(98)00277-5"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1155\/S1064744903000024"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2016.09.075"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2018.12.019"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-012-1770-z"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0227285"}],"container-title":["BJOG: An International Journal of Obstetrics &amp; 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