{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:08:27Z","timestamp":1792793307034,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:00:00Z","timestamp":1727308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005847","name":"Health and Medical Research Fund","doi-asserted-by":"publisher","award":["19181142"],"award-info":[{"award-number":["19181142"]}],"id":[{"id":"10.13039\/501100005847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJOG"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare the performance of cytology, HPV16\/18 genotyping and PAX1\/SOX1 methylation for the triage of high\u2010risk HPV\u2010positive cervical samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Retrospective analyses of archival samples collected from a large\u2010scale prospective randomised controlled trial.<\/jats:p><\/jats:sec><jats:sec><jats:title>Setting\/Sample<\/jats:title><jats:p>HPV\u2010positive women recruited from the general cervical screening population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>403 HPV\u2010positive samples including 113 normal, 173 low\u2010grade cervical intraepithelial neoplasia (LG\u2010CIN), 114 HG\u2010CIN and three cervical cancers. All samples were assessed by liquid\u2010based cytology, HPV genotyping and PAX1\/SOX1 methylation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Main Outcome Measures<\/jats:title><jats:p>AUC (area under the curve), sensitivity and specificity for cytology, HPV16\/18 genotyping and PAX1\/SOX1 methylation for high\u2010grade (HG) premalignant cervical lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PAX1 was more sensitive than cytology and HPV16\/18 genotyping in detecting a HG lesion (CIN2+). The sensitivity for PAX1, SOX1, cytology and HPV16\/18 were 73.5% (95% CI: 65.5\u201381.5), 41.9% (95% CI: 32.9\u201350.8), 48.7% (95% CI: 39.7\u201357.8) and 36.8% (95% CI: 28.0\u201345.5), respectively, and their respective specificities were 70.3% (95% CI: 65.0\u201375.6), 83.6% (95% CI: 79.3\u201387.9), 77.6% (95% CI: 72.8\u201382.5) and 67.1% (95% CI: 61.7\u201372.6), respectively. Overall, PAX1 gave the best AUC at 0.72. Adding SOX1 to PAX1 did not improve the AUC (0.68). Three hundred and twenty\u2010two women who did not have a HG lesion at baseline were followed up for two rounds of screening. Fewer women developed a HG lesion with a normal baseline PAX1 compared to women with a normal baseline cytology or negative HPV16\/18 (8.4% vs. 14.5% and 17.5%, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PAX1 triage for referral to colposcopy in HPV\u2010positive women may be superior to cytology and HPV16\/18 genotyping.<\/jats:p><\/jats:sec>","DOI":"10.1111\/1471-0528.17965","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T04:59:59Z","timestamp":1727413199000},"page":"197-204","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["<scp>PAX1<\/scp>\/<scp>SOX1 DNA<\/scp> Methylation Versus Cytology and <scp>HPV16<\/scp>\/18 Genotyping for the Triage of High\u2010Risk <scp>HPV<\/scp>\u2010Positive Women in Cervical Cancer Screening: Retrospective Analysis of Archival Samples"],"prefix":"10.1111","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4034-7469","authenticated-orcid":false,"given":"Karen K. L.","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology The University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie S.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology The University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lesley S. K.","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology The University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siew Fei","family":"Ngu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology The University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandy M. Y.","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology The University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. Y.","family":"Tse","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynaecology The University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie N. Y.","family":"Cheung","sequence":"additional","affiliation":[{"name":"Department of Pathology, Li Ka Shing Faculty of Medicine The University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hextan Y. 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