{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T08:50:12Z","timestamp":1791881412194,"version":"4.3.4"},"reference-count":51,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009110","name":"Natural Science Foundation of Xinjiang Uygur Autonomous Region","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009110","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BJOG"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Women with polycystic ovary syndrome (PCOS) exhibit a substantially increased risk of miscarriage, yet the underlying mechanisms remain inadequately understood. This study aimed to investigate whether specific gut microbial dysbiosis and metabolic disturbance are associated with and may potentially contribute to endometrial dysfunction and adverse pregnancy outcomes in women with PCOS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>Prospective cohort study integrated with mechanistic experiments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Setting<\/jats:title>\n                    <jats:p>Women's Hospital, School of Medicine, Zhejiang University, China (2022\u20132024).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Population<\/jats:title>\n                    <jats:p>A total of 110 women with PCOS and 110 age\u2010 and body mass index\u2010matched controls were enrolled.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We performed 16S rRNA and metagenomic sequencing of gut microbiota, with untargeted and targeted serum metabolomics. Functional validation was conducted using primary human endometrial stromal cells and a PCOS rat model intervened with\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Parabacteroides merdae<\/jats:italic>\n                      <\/jats:styled-content>\n                      (\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. merdae<\/jats:italic>\n                      <\/jats:styled-content>\n                      ) supplementation or faecal microbiota transplantation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Main Outcome Measures<\/jats:title>\n                    <jats:p>Gut microbiota composition, serum metabolites, endometrial senescence markers, and pregnancy outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Women with PCOS exhibited significantly higher miscarriage rates than controls, accompanied by a marked depletion of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. merdae<\/jats:italic>\n                      <\/jats:styled-content>\n                      abundance and elevated serum levels of branched\u2010chain amino acids, particularly isoleucine. Exogenous isoleucine induced cellular senescence in human endometrial stromal cells in a dose\u2010dependent manner. Restoration of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. merdae<\/jats:italic>\n                      <\/jats:styled-content>\n                      levels in the PCOS rat model resulted in decreased serum isoleucine levels, amelioration of the senescent endometrial phenotype, and reduction in the fetal resorption rate.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These findings suggest that\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. merdae<\/jats:italic>\n                      <\/jats:styled-content>\n                      depletion and the concurrent accumulation of isoleucine may be associated with endometrial senescence and elevated risk of miscarriage, suggesting the possible involvement of a gut microbiota\u2013metabolite pathway in PCOS\u2010related reproductive dysfunction. These results also provide a mechanistic basis for future translational investigations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1471-0528.70266","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T07:55:09Z","timestamp":1779177309000},"page":"2092-2102","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microbial and Metabolic Correlates of Endometrial Dysfunction in Polycystic Ovary Syndrome: A Translational Study"],"prefix":"10.1111","volume":"133","author":[{"given":"Mengyu","family":"Jing","sequence":"first","affiliation":[{"name":"Department of Reproductive Endocrinology, Women's Hospital, School of Medicine Zhejiang University  Zhejiang Hangzhou People's Republic of China"},{"name":"Key Laboratory of Reproductive Genetics (Ministry of Education) Zhejiang University  Zhejiang Hangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, Women's Hospital, School of Medicine Zhejiang University  Zhejiang Hangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiyan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine Zhejiang University  Hangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiya","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Reproductive Endocrinology, Women's Hospital, School of Medicine Zhejiang University  Zhejiang Hangzhou People's Republic of China"},{"name":"Key Laboratory of Reproductive Genetics (Ministry of Education) Zhejiang University  Zhejiang Hangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Reproductive Genetics (Ministry of Education) Zhejiang University  Zhejiang Hangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejing","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine Center Hangzhou Women's Hospital  Zhejiang Hangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghui","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Reproduction People's Hospital of Xinjiang Uygur Autonomous Region  Urumqi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningning","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Reproductive Endocrinology, Women's Hospital, School of Medicine Zhejiang University  Zhejiang Hangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of NCDs Control and Prevention Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8115-8234","authenticated-orcid":false,"given":"Aixia","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Reproductive Endocrinology, Women's Hospital, School of Medicine Zhejiang University  Zhejiang Hangzhou People's Republic of China"},{"name":"Key Laboratory of Reproductive Genetics (Ministry of Education) Zhejiang University  Zhejiang Hangzhou People's Republic of China"},{"name":"Institute of Medical Genetics and Development Zhejiang University  Zhejiang Hangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dew218"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cen.13795"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.4158\/EP15748.DSCPT2"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2016.11.008"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmv029"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgz263"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000036075"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-021-00231-6"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-021-00799-9"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40618-019-01139-x"},{"issue":"1","key":"e_1_2_11_12_1","first-page":"1","article-title":"Effects of Probiotic Supplementation on Hormonal Profiles, Biomarkers of Inflammation and Oxidative Stress in Women With Polycystic Ovary Syndrome: A Randomized, Double\u2010Blind, Placebo\u2010Controlled Trial","volume":"21","author":"Karamali M.","year":"2018","journal-title":"Archives of Iranian Medicine"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.12558"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms251910636"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1239276"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.933110"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.156000"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-021-00199-3"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2023.101026"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2010.02525.x"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.788772"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29912"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-025-00883-8"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-025-03592-z"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.31274"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44318-024-00220-3"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh098"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2006.01008.x"},{"issue":"9","key":"e_1_2_11_29_1","first-page":"653","article-title":"Chinese Expert Consensus on Diagnosis and Management of Recurrent Spontaneous Abortion (2022)","volume":"57","author":"Obstetrics Subgroup, Chinese Society of Obstetrics and Gynecology; Chinese Medical Association","year":"2022","journal-title":"Zhonghua Fu Chan Ke Za Zhi"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2008.02041.x"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/09513590.2023.2219342"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-022-00649-y"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1017147"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.12834"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18646"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2019.01.004"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2024.07.012"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.14434"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202303489"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2222"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.69603"},{"issue":"4","key":"e_1_2_11_42_1","first-page":"7","article-title":"The Endometrium as a Sensor of Embryo Quality","volume":"111","author":"Brosens J. J.","year":"2015","journal-title":"Biology of Reproduction"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-024-03854-6"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deac240"},{"issue":"4","key":"e_1_2_11_45_1","first-page":"201","article-title":"Cytokine Profile in the Endometrium of Normal Fertile and Women With Repeated Implantation Failure","volume":"8","author":"Rajaei S.","year":"2011","journal-title":"Iranian Journal of Immunology"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioaa044"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.31274"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2007-0058"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2014-1292"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gap092"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1017\/S096719942000026X"},{"key":"e_1_2_11_52_1","doi-asserted-by":"crossref","first-page":"931","DOI":"10.2147\/DMSO.S501972","article-title":"Evaluation of Effects of a High\u2010Protein Hypocaloric Diet on Body Composition and Cardio\u2010Metabolic Factors in Women With Polycystic Ovary Syndrome and Overweight or Obesity","volume":"18","author":"Wang F.","year":"2025","journal-title":"Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy"}],"container-title":["BJOG: An International Journal of Obstetrics &amp; Gynaecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1471-0528.70266","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1471-0528.70266","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1471-0528.70266","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T08:18:03Z","timestamp":1791879483000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/1471-0528.70266"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,19]]},"references-count":51,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1111\/1471-0528.70266"],"URL":"https:\/\/doi.org\/10.1111\/1471-0528.70266","archive":["Portico"],"relation":{},"ISSN":["1470-0328","1471-0528"],"issn-type":[{"value":"1470-0328","type":"print"},{"value":"1471-0528","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,19]]},"assertion":[{"value":"2025-11-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}