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Because of this, there is a growing demand for rapid, selective, sensitive, and validated methods for analysis and quantification. Bioactive plant compounds include those with weak estrogenic activity (phytoestrogens), among which are the isoflavones. Some of the beneficial activities that have been attributed to isoflavones are anticarcinogenic activity, the prevention of cardiovascular disease, the improvement of bone health, and antioxidant activity. The objective of this work is to provide an updated review of the methods used in sample preparation and subsequent analysis for the determination of isoflavones in food samples, including both soybean and soy products, as well as other foods with low isoflavone contents. The review focuses on the most common sample preparation techniques used during the last 10 years, including both conventional solvent extraction and other more recent extraction techniques. Separation and detection methods, including current trends in liquid chromatography analysis, such as the use of monolithic columns or ultra\u2010high\u2010pressure liquid chromatography, are also discussed.<\/jats:p>","DOI":"10.1111\/1541-4337.12325","type":"journal-article","created":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T19:03:18Z","timestamp":1515524598000},"page":"391-411","source":"Crossref","is-referenced-by-count":70,"title":["Analysis of Isoflavones in Foods"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7490-8455","authenticated-orcid":false,"given":"Myriam","family":"Bustamante\u2010Rangel","sequence":"first","affiliation":[{"name":"Dept. of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemical Sciences Univ. of Salamanca  Plaza de los Ca\u00eddos s\/n 37008 Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Milagros","family":"Delgado\u2010Zamarre\u00f1o","sequence":"additional","affiliation":[{"name":"Dept. of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemical Sciences Univ. of Salamanca  Plaza de los Ca\u00eddos s\/n 37008 Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"P\u00e9rez\u2010Mart\u00edn","sequence":"additional","affiliation":[{"name":"Dept. of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemical Sciences Univ. of Salamanca  Plaza de los Ca\u00eddos s\/n 37008 Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Encarnaci\u00f3n","family":"Rodr\u00edguez\u2010Gonzalo","sequence":"additional","affiliation":[{"name":"Dept. of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemical Sciences Univ. of Salamanca  Plaza de los Ca\u00eddos s\/n 37008 Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Dom\u00ednguez\u2010\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Dept. of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemical Sciences Univ. of Salamanca  Plaza de los Ca\u00eddos s\/n 37008 Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.3474"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2014.06.007"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/09637480601149350"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2012.02.017"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/157341206776819274"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2013.03.100"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/09637486.2014.917153"},{"key":"e_1_2_7_9_1","first-page":"520","article-title":"Transgenic soybean BRS 243 RR: determination of macronutrients and isoflavones daidzein and genistein by high\u2010performance liquid chromatography (HPLC)","volume":"28","author":"Alezandro MR","year":"2008","journal-title":"Ciencia Tecnol Alime"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1016\/j.foodchem.2010.03.061","article-title":"Method development and validation for isoflavones quantification in coffee","volume":"122","author":"Alves RC","year":"2010","journal-title":"Food Chem"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1093\/jaoac\/86.2.412","article-title":"Fast and easy multiresidue method employing acetonitrile extraction\/partitioning and \u201cdispersive solid\u2010phase extraction\u201d for the determination of pesticide residues in produce","volume":"86","author":"Anastassiades M","year":"2003","journal-title":"J AOAC Intl"},{"key":"e_1_2_7_12_1","volume-title":"Official methods of analysis. 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