{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T11:02:38Z","timestamp":1790247758982,"version":"4.1.0"},"reference-count":107,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Comp Rev Food Sci Food Safe"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Fusarium<\/jats:italic>\n                    species cause yield losses in wheat production through fusarium head blight (FHB) and the associated contamination of regulated mycotoxins, such as trichothecenes and zearalenone. Despite the widespread use of PCR\u2010based molecular approaches for\n                    <jats:italic>Fusarium<\/jats:italic>\n                    detection, quantification, and chemotyping, most primers were developed prior to both modern phylogenetic reclassification and the availability of high\u2010quality genome assemblies, leaving their specificity and robustness largely untested. Existing PCR\u2010 and qPCR\u2010based assays for\n                    <jats:italic>Fusarium<\/jats:italic>\n                    detection in wheat were reviewed and re\u2010evaluated in silico using a curated genome panel. Of 53 species\u2010specific primer pairs, 14 (26.4%) achieved high\u2010specificity grades (A\u2013B), whereas 25 (47.2%) were lower performing (D\u2013E), mainly due to cross\u2010reactivity or inconsistent target amplification. Chemotype assays targeting TRI and ZEN genes showed stronger agreement with reported chemotypes, especially for informative TRI loci such as\n                    <jats:italic>Tri3<\/jats:italic>\n                    ,\n                    <jats:italic>Tri7<\/jats:italic>\n                    , and\n                    <jats:italic>Tri12<\/jats:italic>\n                    . To support improved qPCR assay design and reporting, we propose FusaMIQE, a\n                    <jats:italic>Fusarium<\/jats:italic>\n                    \u2010adapted framework based on MIQE 2.0 guidelines, tailored to the specific challenges of\n                    <jats:italic>Fusarium<\/jats:italic>\n                    diagnostics in wheat. Together, this manuscript provides the first in silico assessment of PCR\/qPCR primers as diagnostic tools for FHB pathogens and associated recommendations for good practice (FusaMIQE) of\n                    <jats:italic>Fusarium<\/jats:italic>\n                    diagnostics in wheat.\n                  <\/jats:p>","DOI":"10.1111\/1541-4337.70612","type":"journal-article","created":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T03:30:39Z","timestamp":1787974239000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Revisiting the PCR\u2010Based Molecular Approaches for Mycotoxigenic\n                    <i>Fusarium<\/i>\n                    Species Detection and Quantification in Wheat"],"prefix":"10.1111","volume":"25","author":[{"given":"Kavitha","family":"Vijeandran","sequence":"first","affiliation":[{"name":"Faculty of Engineering and Applied Sciences Cranfield University  Cranfield UK"},{"name":"Magan Centre of Applied Mycology Cranfield University  Cranfield UK"},{"name":"Institute for Global Food Security, School of Biological Sciences Queen's University Belfast  Belfast UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey","family":"Larionov","sequence":"additional","affiliation":[{"name":"Faculty of Engineering and Applied Sciences Cranfield University  Cranfield UK"},{"name":"Department of Genomic Medicine University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Cervini","sequence":"additional","affiliation":[{"name":"Magan Centre of Applied Mycology Cranfield University  Cranfield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrina","family":"Campbell","sequence":"additional","affiliation":[{"name":"Institute for Global Food Security, School of Biological Sciences Queen's University Belfast  Belfast UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Walkowiak","sequence":"additional","affiliation":[{"name":"Grain Research Laboratory Canadian Grain Commission  Winnipeg Manitoba Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matias","family":"Pasquali","sequence":"additional","affiliation":[{"name":"Department of Food, Environmental and Nutritional Sciences University of Milan  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Richard\u2010Forget","sequence":"additional","affiliation":[{"name":"INRAE, Mycology and Food Safety (MycSA)  Villenave d'Ornon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Life Sciences University of Bath  Bath UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindy Joy","family":"Rose","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology Stellenbosch University  Stellenbosch South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3470-9803","authenticated-orcid":false,"given":"Carol","family":"Verheecke\u2010Vaessen","sequence":"additional","affiliation":[{"name":"Magan Centre of Applied Mycology Cranfield University  Cranfield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"e_1_2_12_2_1","unstructured":"Agriculture and Horticulture Development Board.2025. \u201cSeedborne Diseases of Cereals: Tests and 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