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In this study, we tested different fixatives\u2014phosphate\u2010buffered paraformaldehyde 4% (<jats:styled-content style=\"fixed-case\">PP<\/jats:styled-content>), Carnoy's fluid (<jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>), Kahle's solution (<jats:styled-content style=\"fixed-case\">KS<\/jats:styled-content>), ethanol in different concentrations, and ethanol associated to<jats:styled-content style=\"fixed-case\">PP<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>, time of fixation and histological processes for dipteran larvae's tissue, aiming to develop a sample preparation protocol for IHC application. A suitable fixation was achieved using<jats:styled-content style=\"fixed-case\">PP<\/jats:styled-content>for 12 and 24\u00a0h,<jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>for 3\u00a0h, 70% ethanol for 19\u00a0days, and 70% ethanol\/<jats:styled-content style=\"fixed-case\">CF<\/jats:styled-content>for 2\u00a0h\/3\u00a0h. Postfixation using negative pressure, two immersions in xylene for 30\u00a0min each, and one in xylene plus paraffin for 45\u00a0min increased tissue preservation. An immunohistochemical test for cocaine detection was performed using monoclonal benzoylecgonine antibody from mouse, peroxidase\u2010conjugated anti\u2010mouse IgG and visualized by 3,3\u2032\u2010diaminobenzidine method showed these histological procedures didn't compromise antigenicity.<\/jats:p>","DOI":"10.1111\/1556-4029.12140","type":"journal-article","created":{"date-parts":[[2013,5,17]],"date-time":"2013-05-17T20:28:03Z","timestamp":1368822483000},"page":"1015-1021","source":"Crossref","is-referenced-by-count":8,"title":["Standardization of Histological Procedures for the Detection of Toxic Substances by Immunohistochemistry in Dipteran Larvae of Forensic Importance"],"prefix":"10.1111","volume":"58","author":[{"given":"Carina M.","family":"Souza","sequence":"first","affiliation":[{"name":"Department of Animal Biology IB UNICAMP Distrito de Bar\u00e3o Geraldo Campinas S\u00e3o Paulo CEP 13083\u2010970 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina G. 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