{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T17:40:46Z","timestamp":1695490846672},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,10,22]],"date-time":"2013-10-22T00:00:00Z","timestamp":1382400000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A bead\u2010based liquid hybridization assay, <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>uminex<jats:sup>\u00ae<\/jats:sup> 100\u2122, was used to identify four pathogenic bacteria, <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>acillus anthracis<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>lostridium botulinum<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>rancisella tularensis<\/jats:italic> subsp. <jats:italic>tularensis<\/jats:italic>, and <jats:italic><jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>ersinia pestis<\/jats:italic>, and several close relatives. Hybridization between <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010amplified target sequences and probe sequences (located within the 23S ribosomal <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> gene <jats:italic>rrl<\/jats:italic> and the genes related to the toxicity of each bacterium) was detected in single\u2010probe or multiple\u2010probe assays, depending on the organism. The lower limits of detection (<jats:styled-content style=\"fixed-case\">LLD<\/jats:styled-content>s) for the probes ranged from 0.1 to 10\u00a0ng. Sensitivity was improved using lambda exonuclease to digest the noncomplementary target strand. All contributors in 33 binary, ternary, and quaternary mixtures in which all components were present in a 1:1 ratio were identified with an 80% success rate. Twenty\u2010eight binary mixtures in which the two components were combined in various ratios were further studied. All target sequences were detected, even when the minor component was overshadowed by a tenfold excess of the major component.<\/jats:p>","DOI":"10.1111\/1556-4029.12283","type":"journal-article","created":{"date-parts":[[2013,10,22]],"date-time":"2013-10-22T16:12:40Z","timestamp":1382458360000},"page":"15-33","source":"Crossref","is-referenced-by-count":7,"title":["Detection of Anthrax and Other Pathogens Using a Unique Liquid Array Technology"],"prefix":"10.1111","volume":"59","author":[{"given":"Andrew J.","family":"Schweighardt","sequence":"first","affiliation":[{"name":"Graduate School and University Center The City University of New York 365 Fifth Avenue New York NY 10016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda","family":"Battaglia","sequence":"additional","affiliation":[{"name":"Office of Chief Medical Examiner The City of New York 421 East 26th Street New York NY 10016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret M.","family":"Wallace","sequence":"additional","affiliation":[{"name":"Department of Sciences John Jay College of Criminal Justice The City University of New York 445 West 59th Street New York NY 10019"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10,22]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"1","volume-title":"Medical aspects of biological warfare","author":"Martin J","year":"2007"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4258-4265.2000"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00238-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.4.583-607.2005"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2006.11.004"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001280278"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac034722v"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac035365r"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1556-4029.2011.01708.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-19"},{"key":"e_1_2_6_12_1","unstructured":"http:\/\/www.bt.cdc.gov\/agent\/agentlist-category.asp(accessed September 7 2011)."},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01586"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001271"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1499"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/35097083"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01582"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1830"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007041"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020206"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/31159"},{"key":"e_1_2_6_22_1","volume-title":"Bergey's manual of systematic bacteriology, volume I: the Archaea and the deeply branching phototrophic bacteria","author":"Garrity G","year":"2001"},{"key":"e_1_2_6_23_1","unstructured":"http:\/\/blast.ncbi.nlm.nih.gov\/Blast.cgi(accessed March 13 2010)."},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-512-1_1"},{"key":"e_1_2_6_25_1","unstructured":"http:\/\/align.genome.jp\/(accessed January 17 2010)."},{"key":"e_1_2_6_26_1","first-page":"365","volume-title":"Bioinformatics methods and protocols: methods in molecular biology","author":"Rozen S","year":"2000"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.2144\/04372ST03"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.14.4008"},{"key":"e_1_2_6_29_1","unstructured":"http:\/\/www.chem.agilent.com\/Library\/usermanuals\/Public\/G2938-90014_KitGuideDNA1000Assay_ebook.pdf(accessed September 8 2011)."},{"key":"e_1_2_6_30_1","unstructured":"http:\/\/www.luminexcorp.com\/prod\/groups\/public\/documents\/lmnxcorp\/oligonucleotide-coupling-proto.pdf(accessed September 3 2012)."},{"key":"e_1_2_6_31_1","volume-title":"Pathogen detection using the Luminex multi\u2010analyte system [dissertation]","author":"Schweighardt AJ","year":"2012"},{"key":"e_1_2_6_32_1","first-page":"200","volume-title":"Bergey's manual of systematic bacteriology","author":"Sjostedt A","year":"2004"},{"key":"e_1_2_6_33_1","unstructured":"http:\/\/www.ncbi.nlm.nih.gov\/protein\/89143742(accessed September 3 2012)."},{"key":"e_1_2_6_34_1","unstructured":"http:\/\/www.ncbi.nlm.nih.gov\/nucleotide\/89143280?report=gbwithparts&from=489874&to=490044&RID=3S4KBB21012(accessed September 4 2012)."},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00212-09"},{"key":"e_1_2_6_36_1","first-page":"114","article-title":"Rapid screening for 31 mutations and polymorphisms in the cystic fibrosis transmembrane conductance regulator gene by Luminex xMAP suspension array","volume":"114","author":"Dunbar S","year":"2005","journal-title":"Methods Mol Med"},{"issue":"12","key":"e_1_2_6_37_1","doi-asserted-by":"crossref","first-page":"4870","DOI":"10.1128\/AEM.64.12.4870-4876.1998","article-title":"A new approach to utilize PCR\u2010single\u2010strand\u2010conformation polymorphism for 16S rRNA gene\u2010based microbial community analysis","volume":"64","author":"Schwieger F","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.15.3057"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2009.12.001"}],"container-title":["Journal of Forensic Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1556-4029.12283","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1556-4029.12283","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T17:13:18Z","timestamp":1695489198000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1556-4029.12283"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,22]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1111\/1556-4029.12283"],"URL":"https:\/\/doi.org\/10.1111\/1556-4029.12283","archive":["Portico"],"relation":{},"ISSN":["0022-1198","1556-4029"],"issn-type":[{"value":"0022-1198","type":"print"},{"value":"1556-4029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,22]]}}}