{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:11:31Z","timestamp":1784592691896,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,2,6]],"date-time":"2014-02-06T00:00:00Z","timestamp":1391644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In order to devise a better forensic test for diatoms, the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> binding ability of the diatom frustule constructing by silica, in the presence of chaotropic ions were utilized. It was proved that the diatoms were able to be captured via \u03bb<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> using silica\u2010coated magnetic beads (Mag beads), followed by isolation and purification from the Mag beads as a solid phase by substituting the chaotropic agent with ultrapure water. Five cases of drowning, three in freshwater and two in seawater, were applied to the present method and similar results as the usual diatom test were obtained. Specimens of lung and other organs were rendered clearly visible, with elimination of foreign impurities. The present method appears applicable for detection of diatoms indirectly using <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> amplification of bound <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> or directly staining of the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/1556-4029.12390","type":"journal-article","created":{"date-parts":[[2014,2,6]],"date-time":"2014-02-06T22:54:48Z","timestamp":1391727288000},"page":"779-784","source":"Crossref","is-referenced-by-count":5,"title":["An Improved Method for the Diatom Test Utilizing <scp>DNA<\/scp> Binding Ability of Silica"],"prefix":"10.1111","volume":"59","author":[{"given":"Yasuhisa","family":"Seo","sequence":"first","affiliation":[{"name":"Department of Forensic Medicine Oita University Faculty of Medicine Hasama\u2010machi Yufu City Oita 879\u20105593 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Ichida","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine Oita University Faculty of Medicine Hasama\u2010machi Yufu City Oita 879\u20105593 Japan"},{"name":"Oita Coast Guard Office 7th Regional Coast Guard Headquarters Chihama Kaiwara Oita City Oita 870\u20100107 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine Oita University Faculty of Medicine Hasama\u2010machi Yufu City Oita 879\u20105593 Japan"},{"name":"Oita Coast Guard Office 7th Regional Coast Guard Headquarters Chihama Kaiwara Oita City Oita 870\u20100107 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohji","family":"Kuroki","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine Oita University Faculty of Medicine Hasama\u2010machi Yufu City Oita 879\u20105593 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuko","family":"Kishida","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine Oita University Faculty of Medicine Hasama\u2010machi Yufu City Oita 879\u20105593 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,6]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"274","article-title":"Der Nachweis der aspirierten Ertr\u00e4nkungs Fl\u00fcssigkeit als Kriterium des Todes durch Ertrinken","volume":"27","author":"Revenstorf W","year":"1904","journal-title":"Vjschr Gerichtl Med"},{"issue":"2","key":"e_1_2_5_3_1","first-page":"45","article-title":"Medico\u2010legal problems in death by drowning. 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