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We assessed the time and temperature\u2010dependent changes in cytochrome <jats:italic>c<\/jats:italic> oxidase (<jats:styled-content style=\"fixed-case\">CCO<\/jats:styled-content>) activity and cyanide concentration in various organs of mice succumbing to cyanide. Immediately after death, excised mice organs and mice cadavers were stored at room temperature (35\u00b0C\u00a0\u00b1\u00a05\u00b0C) or in frozen storage (\u221220\u00b0C\u00a0\u00b1\u00a02\u00b0C). At various times after death, <jats:styled-content style=\"fixed-case\">CCO<\/jats:styled-content> activity and cyanide concentrations were measured in excised mice organs or organs removed from mice cadavers. The study revealed that (i) measuring both the biomarkers in mice cadavers was more reliable compared to excised mice organs, (ii) measuring temporal <jats:styled-content style=\"fixed-case\">CCO<\/jats:styled-content> activity and cyanide concentration in vital organs from mice cadavers (room temperature) was reliable up to 24\u00a0h, and (iii) <jats:styled-content style=\"fixed-case\">CCO<\/jats:styled-content> activity in the brain and lungs and cyanide concentration in organs from mice cadavers (frozen) were measurable beyond 21\u00a0days. This study will be helpful in postmortem determination of cyanide poisoning.<\/jats:p>","DOI":"10.1111\/1556-4029.12614","type":"journal-article","created":{"date-parts":[[2014,11,24]],"date-time":"2014-11-24T18:06:23Z","timestamp":1416852383000},"source":"Crossref","is-referenced-by-count":6,"title":["Time\u2010 and Temperature\u2010Dependent Changes in Cytochrome <i>c<\/i> Oxidase Activity and Cyanide Concentration in Excised Mice Organs and Mice Cadavers"],"prefix":"10.1111","volume":"60","author":[{"given":"Poonam","family":"Singh","sequence":"first","affiliation":[{"name":"Division of Pharmacology and Toxicology Defense Research and Development Establishment Gwalior 474002 Madhya Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooja","family":"Rao","sequence":"additional","affiliation":[{"name":"Division of Pharmacology and Toxicology Defense Research and Development Establishment Gwalior 474002 Madhya Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiv K.","family":"Yadav","sequence":"additional","affiliation":[{"name":"Division of Pharmacology and Toxicology Defense Research and Development Establishment Gwalior 474002 Madhya Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niranjan L.","family":"Gujar","sequence":"additional","affiliation":[{"name":"Division of Pharmacology and Toxicology Defense Research and Development Establishment Gwalior 474002 Madhya Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravindra M.","family":"Satpute","sequence":"additional","affiliation":[{"name":"Division of Pharmacology and Toxicology Defense Research and Development Establishment Gwalior 474002 Madhya Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul","family":"Bhattacharya","sequence":"additional","affiliation":[{"name":"Division of Pharmacology and Toxicology Defense Research and Development Establishment Gwalior 474002 Madhya Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,24]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"272","volume-title":"Medical aspects of chemical and biological warfare. 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