{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:17:49Z","timestamp":1790745469138,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,30]],"date-time":"2015-04-30T00:00:00Z","timestamp":1430352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of the Interior of the Republic of Croatia"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2015,7]]},"abstract":"Abstract<\/jats:title>Forensic evidence samples are routinely found as stains on various substrates, which may contain substances known to inhibit polymerase chain reaction (PCR<\/jats:styled-content>). The goal of this study was to evaluate post\u2010Chelex\u00ae<\/jats:sup>100 purification using powdered activated carbon (PAC<\/jats:styled-content>). Mock crime scene DNA<\/jats:styled-content> extracts were analyzed using quantitative PCR<\/jats:styled-content> and short tandem repeat (STR<\/jats:styled-content>) profiling to test the DNA<\/jats:styled-content> recovery and inhibitor removal using PAC<\/jats:styled-content> with those of the Amicon\u00ae<\/jats:sup>Ultra 100K. For extracted bloodstains on soil and wood substrates, PAC<\/jats:styled-content> and Amicon\u00ae<\/jats:sup>Ultra 100K generated similar DNA<\/jats:styled-content> yield and quality. Moreover, the two methods significantly decreased the concentration of humic substances and tannins compared to nonpurified extracts (p\u00a0<\/jats:italic><\u00a0<\/jats:italic>0.001). In instances where extracts contained indigo dye (bloodstains on denim), Amicon\u00ae<\/jats:sup>Ultra 100K performed better than PAC<\/jats:styled-content> due to improved amplifiability. Efficient adsorption of humic substances and tannins, which are common inhibitors, indicates PAC<\/jats:styled-content>'s potential application in the purification of high\u2010template DNA<\/jats:styled-content> extracts.<\/jats:p>","DOI":"10.1111\/1556-4029.12773","type":"journal-article","created":{"date-parts":[[2015,4,30]],"date-time":"2015-04-30T18:40:09Z","timestamp":1430419209000},"page":"1012-1015","source":"Crossref","is-referenced-by-count":5,"title":["Powdered Activated Carbon: An Alternative Approach to Genomic DNA Purification"],"prefix":"10.1111","volume":"60","author":[{"given":"Lucija","family":"Barbari\u0107","sequence":"first","affiliation":[{"name":"Ivan Vu\u010deti\u0107 Forensic Science Center General Police Directorate Ministry of the Interior of the Republic of Croatia Ilica 335 10000 Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Ba\u010di\u0107","sequence":"additional","affiliation":[{"name":"Ivan 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