{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T22:18:25Z","timestamp":1759961905148,"version":"3.37.3"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,8,6]],"date-time":"2015-08-06T00:00:00Z","timestamp":1438819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australia Research Council","doi-asserted-by":"crossref","award":["DP110101364"],"award-info":[{"award-number":["DP110101364"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2015,11]]},"abstract":"Abstract<\/jats:title>The successful extraction of DNA<\/jats:styled-content> from historical or ancient animal bone is important for the analysis of discriminating genetic markers. Methods used currently rely on the digestion of bone with EDTA<\/jats:styled-content> and proteinase K, followed by purification with phenol\/chloroform and silica bed binding. We have developed a simple concentrated hydrochloric acid\u2010based method that precludes the use of phenol\/chloroform purification and can lead to a several\u2010fold increase in DNA<\/jats:styled-content> yield when compared to other commonly used methods. Concentrated hydrochloric acid was shown to dissolve most of the undigested bone and allowed the efficient recovery of DNA<\/jats:styled-content> fragments <100 bases in length. This method should prove useful for the recovery of DNA<\/jats:styled-content>s from highly degraded animal bone, such as that found in historical or ancient samples.<\/jats:p>","DOI":"10.1111\/1556-4029.12846","type":"journal-article","created":{"date-parts":[[2015,8,6]],"date-time":"2015-08-06T14:17:02Z","timestamp":1438870622000},"page":"1553-1557","source":"Crossref","is-referenced-by-count":7,"title":["A Concentrated Hydrochloric Acid\u2010based Method for Complete Recovery of DNA<\/scp> from Bone"],"prefix":"10.1111","volume":"60","author":[{"given":"Leon","family":"Huynen","sequence":"first","affiliation":[{"name":"Environmental Futures Research Institute Griffith University 170 Kessels Road Nathan Qld 4111 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Lambert","sequence":"additional","affiliation":[{"name":"Environmental Futures Research Institute Griffith University 170 Kessels Road Nathan Qld 4111 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,8,6]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1366-2120(08)70035-7"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1556-4029.12323"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1258\/msl.2012.012026"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAF.0b013e3182519479"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.22057"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.21647"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-461-2_13"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2011.08.004"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2009.10.014"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.21193"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.247"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2013.05.002"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-516-9_2"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsigen.2007.02.006"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00414-011-0590-5"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1314445110"},{"issue":"3","key":"e_1_2_5_18_1","first-page":"701","article-title":"Authenticity in ancient DNA studies","volume":"18","author":"Gilbert MT","year":"2006","journal-title":"Med Secoli"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.2.378"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050732"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(01)00179-X"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503718102"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.038"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1224344"}],"container-title":["Journal of Forensic Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2F1556-4029.12846","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1556-4029.12846","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T07:55:53Z","timestamp":1694937353000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1556-4029.12846"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8,6]]},"references-count":23,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1111\/1556-4029.12846"],"URL":"https:\/\/doi.org\/10.1111\/1556-4029.12846","archive":["Portico"],"relation":{},"ISSN":["0022-1198","1556-4029"],"issn-type":[{"type":"print","value":"0022-1198"},{"type":"electronic","value":"1556-4029"}],"subject":[],"published":{"date-parts":[[2015,8,6]]}}}