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The oral fluid was applied to a Whatman 903 grade paper and submitted to a drying time of 2.5\u00a0h. The extraction procedure was optimized by chemometric approach using simplex centroid design. Spots were extracted with a mixture of acetonitrile, buffer, and methanol. Calibration curves covered a linear concentration range of 40\u2013500\u00a0ng\/<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content>. Validation parameters of linearity, precision, accuracy, selectivity, carryover, matrix effects, and stability were evaluated and showed satisfactory results. Spot homogeneity was also satisfactory, with less than 15% of deviation from nominal concentration. Spot volume did not influence accuracy when less than 100\u00a0\u03bcL of the sample was applied to the spot. 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