{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:19:00Z","timestamp":1788279540310,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T00:00:00Z","timestamp":1606089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Historically, blood alcohol concentration (BAC) studies utilized a 1% concentration of the preservative sodium fluoride (NaF), leaving an information gap supporting usage of lower concentrations of NaF to preserve ethanol. As many forensic laboratories utilize Becton, Dickinson and Company 6\u2010mL gray\u2010top tubes (0.25% NaF), statistical comparisons were conducted to determine whether significant differences exist between BAC values obtained from 6\u2010mL tubes versus 10\u2010mL tubes (1% NaF). Whole blood was spiked at three concentrations, (0.04, 0.08, and 0.15\u00a0g\/100\u00a0mL) and aliquoted into tubes at \u201clow,\u201d \u201cmedium,\u201d and \u201chigh\u201d fill volumes. Tubes were split into refrigerated or ambient storage and analyzed after 1, 3, 5, 7, and 30\u00a0days, using headspace gas chromatography. Each 6\u2010mL and 10\u2010mL tube pair, prepared, stored, and analyzed under identical conditions, was compared by t\u2010test (95% confidence level). For refrigerated tubes, 32 of 45\u2010tube pairs did not reject the null hypothesis (that 6\u2010mL and 10\u2010mL tubes yield equivalent BACs), and 31 of 45 ambient stored tube pairs did not reject the null hypothesis. Analysis of variance (ANOVA) found no significant differences between 6\u2010mL and 10\u2010mL gray\u2010top tubes for 0.04 and 0.15\u00a0g\/100\u00a0mL concentrations over 30\u00a0days; significant differences were observed for 0.08\u00a0g\/100\u00a0mL concentration tubes, which warrants further study. Paired t\u2010tests of grouped samples found no significant differences between 6\u2010mL and 10\u2010mL tubes at any concentration.<\/jats:p>","DOI":"10.1111\/1556-4029.14632","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T05:19:26Z","timestamp":1606195166000},"page":"687-693","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Statistical comparisons of blood alcohol samples from 6\u2010mL and 10\u2010mL grey\u2010top tubes"],"prefix":"10.1111","volume":"66","author":[{"given":"Maria L.","family":"Olds","sequence":"first","affiliation":[{"name":"Fort Worth Police Department Crime Laboratory Fort Worth TX 76103 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Naquin","sequence":"additional","affiliation":[{"name":"Fort Worth Police Department Crime Laboratory Fort Worth TX 76103 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,23]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"Recall Notification \u2013 BD vacutainer fluoride tubes for blood alcohol determinations.2019.https:\/\/www.bd.com\/en\u2010us\/support\/recall\u2010notifications\/recall\u2010notification\u2010bd\u2010vacutainer\u2010fluoride\u2010tubes\u2010for\u2010blood\u2010alcohol\u2010determinations. Accessed 18 Feb 2020."},{"key":"e_1_2_6_3_1","unstructured":"Becton Dickinson Life Sciences.Preanalytical Systems product catalog.https:\/\/www.bd.com\/resource.aspx?IDX=7220. Accessed 18 Feb 2020."},{"key":"e_1_2_6_4_1","unstructured":"Texas Forensic Science Commission.Becton Dickinson (BD) tube recall memorandum.https:\/\/www.txcourts.gov\/media\/1444363\/bd\u2010memorandum\u2010070819\u20105.pdf. Accessed 20 Feb 2020."},{"key":"e_1_2_6_5_1","unstructured":"Texas District & County Attorneys Association.Blood collection tube recall.2019.https:\/\/www.tdcaa.com\/blood\u2010collection\u2010tube\u2010recall\/. Accessed 29 Apr 2020."},{"key":"e_1_2_6_6_1","unstructured":"BoydD.Could vials used by Fort Worth police alter blood alcohol results? Some experts say yes. The Fort Worth Star\u2010Telegram.2019.https:\/\/www.star\u2010telegram.com\/news\/local\/fort\u2010worth\/article232165692.html. 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