{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T15:22:44Z","timestamp":1780413764212,"version":"3.54.1"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T00:00:00Z","timestamp":1623196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sampling of healthy multi\u2010rooted teeth is recommended for the genetic identification of human skeletal remains. However, this may not always be possible, as in the reported case consisting of an isolated human cranium found in an aggregate crushing and processing plant in Piedmont, Northwest Italy. The cranium displayed significant weathering, suggesting a post\u2010mortem interval of several years, and was edentulous with the exception of the apical root fragment of the upper left canine, consequence of an antemortem horizontal fracture. Prolonged decalcification of the root fragment followed by powder\u2010free DNA extraction from ~10\u00a0mg of root tip tissue led to the recovery of &gt;10\u00a0ng of high molecular weight human DNA, in comparison with ~0.01\u00a0ng of DNA per mg of bone powder obtained from the petrous portion of the temporal bone. Quantity and quality of DNA isolated from apical tooth tissue enabled multiple genotyping, including a reportable female STR profile, mitochondrial DNA analysis, and ancestry\u2010informative insertion\/deletion polymorphisms. Although the cranium remained unidentified after DNA comparisons, our findings confirm that apical tooth tissue is a promising source of DNA, easily obtained through a powder\u2010free extraction protocol. Results also indicate that root tips should not be overlooked in challenging identification cases, even in the presence of compromised tooth specimens.<\/jats:p>","DOI":"10.1111\/1556-4029.14748","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T14:52:08Z","timestamp":1623250328000},"page":"1973-1979","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Sweet tooth: DNA profiling of a cranium from an isolated retained root fragment"],"prefix":"10.1111","volume":"66","author":[{"given":"Elena","family":"Chierto","sequence":"first","affiliation":[{"name":"Department of Public Health Sciences and Pediatrics University of Turin Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greta","family":"Cena","sequence":"additional","affiliation":[{"name":"Department of Public Health Sciences and Pediatrics University of Turin Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Mann","sequence":"additional","affiliation":[{"name":"John A. 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