{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T10:31:05Z","timestamp":1706783465846},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T00:00:00Z","timestamp":1624320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Since the accuracy of headspace gas chromatographic analysis of blood for ethanol concentration has been so well established over the past several decades, it has become commonplace in court proceedings to attack preanalytical handling of the blood samples including the lack of measuring sample temperature prior to sample preparation. The impact on measured ethanol concentration of allowing refrigerated (~4\u2103) samples varying amounts of time to equilibrate with room temperature, 24, 4, 3, 2, and 1\u00a0h, prior to sample preparation was evaluated. Samples were diluted 1:10 with an internal standard using a diluter\/dispenser and analyzed using headspace gas chromatography. The mean ethanol concentration measured for the sixteen samples at each of the five equilibration times was 0.153\u00a0g\/dl. The <jats:italic>F<\/jats:italic>\u2010critical from the one\u2010way ANOVA was 2.4937. The calculated <jats:italic>F<\/jats:italic> value was 0.4209. Additionally, the effect on measured ethanol concentration of having calibrators at different temperatures than case samples was investigated. Three groups were analyzed: all calibrators, controls, and samples given 24\u00a0h to equilibrate with room temperature, all calibrators, controls, and samples prepared immediately after removal from refrigeration, and calibrators sampled immediately after removal from refrigerator with samples and controls allowed 24\u00a0h to equilibrate with room temperature. The mean ethanol concentration measured for the thirty blood samples in each of the three groups was 0.197\u00a0g\/dl. The <jats:italic>F<\/jats:italic>\u2010critical from the one\u2010way ANOVA was 3.1013. The calculated <jats:italic>F<\/jats:italic> value was 0.0188. Measured ethanol concentrations were insensitive to the variations in preanalytical conditions evaluated in this study.<\/jats:p>","DOI":"10.1111\/1556-4029.14787","type":"journal-article","created":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T11:10:57Z","timestamp":1624360257000},"page":"2478-2483","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The effect of sample temperature variations during sample preparation on measured blood ethanol concentration"],"prefix":"10.1111","volume":"66","author":[{"given":"Patrick Allan","family":"Kosecki","sequence":"first","affiliation":[{"name":"Scottsdale Police Department Crime Laboratory Scottsdale AZ USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phillip","family":"Brooke","sequence":"additional","affiliation":[{"name":"Scottsdale Police Department Crime Laboratory Scottsdale AZ USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"Canonico","sequence":"additional","affiliation":[{"name":"Scottsdale Police Department Crime Laboratory Scottsdale AZ USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,6,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01218048"},{"key":"e_1_2_7_3_1","first-page":"65","volume-title":"Organization, automation of laboratories \u2013 biologic prospective","author":"Kolb B","year":"1970"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/12.5.279"},{"issue":"1","key":"e_1_2_7_5_1","first-page":"136","article-title":"A simple method using head\u2010space gas for determination of blood alcohol by gas chromatography","volume":"14","author":"Glendening BL","year":"1969","journal-title":"J Forensic Sci"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS12748J"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/87.1.79"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jflm.2018.06.003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/002580249103100307"},{"issue":"2","key":"e_1_2_7_10_1","first-page":"3","article-title":"Contamination of blood specimens for alcohol analysis during collection","volume":"4","author":"Dubowski KM","year":"1983","journal-title":"Abstr Rev Alcohol Driving"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/30.5.317"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1258\/rsmmsl.43.3.241"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkv068"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1556\u20104029.14675"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkaa056"},{"key":"e_1_2_7_16_1","unstructured":"Certificate of analysis.https:\/\/www.cerilliant.com\/shoponline\/COA.aspx?itemno=461ae6d3\u20107454\u2010459c\u2010a4f9\u20102b29dbad9b4e&lotno=FN05311902. 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