{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T03:43:37Z","timestamp":1793418217910,"version":"4.5.0"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T00:00:00Z","timestamp":1629244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Metomidate and etomidate belong to the non\u2010barbiturate imidazole family of sedative\u2013hypnotics and elicit little analgesic action when used alone. Metomidate, in particular, has little analgesic activity in humans and is, therefore, used for veterinary purposes. In 2019, a Korean woman in her twenties was found unconscious in a motel bath and eventually died. Etomidate, alprazolam, escitalopram, and metomidate were detected in the postmortem specimens. To our knowledge, this is the first case of human metomidate abuse reported in the Republic of Korea. In this research, a simple and reliable method was developed for the analysis of metomidate and etomidate in human blood samples using liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS). Blood samples were deproteinized with acetonitrile, filtered, and analyzed by LC\u2013MS\/MS. Linear calibration curves were obtained with six concentrations ranging from 1 to 50\u00a0ng\/ml for metomidate and 10 to 500\u00a0ng\/ml for etomidate. The method was validated by assessing the selectivity, linearity, limit of detection (LOD), limit of quantitation (LOQ), intra\u2010 and inter\u2010day precision and accuracy, matrix effect, and stability and successfully applied to the analysis of metomidate and etomidate in human blood samples. In a postmortem case, the concentrations of metomidate and etomidate were found to be 8 and 110\u00a0ng\/ml in femoral blood and 6 and 210\u00a0ng\/ml in cardiac blood, respectively.<\/jats:p>","DOI":"10.1111\/1556-4029.14823","type":"journal-article","created":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T05:53:36Z","timestamp":1629266016000},"page":"2532-2538","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Fast and reliable analysis of veterinary metomidate and etomidate in human blood samples by liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) in a postmortem case"],"prefix":"10.1111","volume":"66","author":[{"given":"Hyesun","family":"Yum","sequence":"first","affiliation":[{"name":"Forensic Toxicology and Chemistry Division Seoul Institute National Forensic Service Seoul South Korea"},{"name":"Department of Pharmaceutical Analysis College of Pharmacy Chung\u2010Ang University Seoul 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