{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T08:20:58Z","timestamp":1722500458345},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T00:00:00Z","timestamp":1640822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Forensic Sciences"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Thiamylal is an ultrashort\u2010acting barbiturate used for intravenous administration or general anesthesia induction. However, some cases of poisoning and suicide with thiamylal administration have been reported. Additionally, there are few reports on its analysis in the organs and adipose tissue, which requires purification by column chromatography and evaporation. A rapid and sensitive method was developed for quantifying thiamylal and its metabolite, secobarbital, in the adipose tissue, serum, and liver using liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS). Samples were prepared using modified QuEChERS extraction. For adipose tissue samples, an acetonitrile\u2013hexane partitioning step was added to the extraction. This method was applied to investigate a suspected self\u2010poisoning autopsy case. The quantitation accuracy for thiamylal added to porcine pericardial fat (0.18\u00a0\u00b5g\/g), human serum (0.015\u00a0\u00b5g\/mL), and porcine liver (0.18\u00a0\u00b5g\/g) was 103%, 113%, and 95.3%, respectively. The quantitation limits calculated for porcine pericardial fat, human serum, and porcine liver at a signal\u2010to\u2010noise ratio of 10 were 0.06\u00a0\u00b5g\/g, 0.005\u00a0\u00b5g\/mL, and 0.06\u00a0\u00b5g\/g, respectively. In addition, the thiamylal and secobarbital levels in the forensic autopsy case were 140 and 1.5\u00a0\u00b5g\/g, respectively, in myocardial fat; 3.5\u20134.9 and 0.12\u20130.20\u00a0\u00b5g\/mL, respectively, in serum; and 6.2\u201342 and 0.58\u20131.1\u00a0\u00b5g\/g, respectively, in liver tissue. Thiamylal is especially distributed in the adipose tissue. The thiamylal\u2010to\u2010fat ratio may help estimate the time from administration to death. The developed modified QuEChERS extraction method with acetonitrile\u2013hexane partitioning is suitable for analyzing hydrophobic compounds, such as thiamylal, in the adipose tissue.<\/jats:p>","DOI":"10.1111\/1556-4029.14965","type":"journal-article","created":{"date-parts":[[2021,12,31]],"date-time":"2021-12-31T07:19:50Z","timestamp":1640935190000},"page":"1241-1249","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Quantitative analysis of thiamylal and its metabolite secobarbital using liquid chromatography\u2013tandem mass spectrometry in adipose tissue, serum, and liver"],"prefix":"10.1111","volume":"67","author":[{"given":"Naoki","family":"Yoshioka","sequence":"first","affiliation":[{"name":"Hyogo Prefectural Institute of Public Health Science  Kakogawa Japan"},{"name":"Division of Legal Medicine Department of Environmental Health and Safety Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Migiwa","family":"Asano","sequence":"additional","affiliation":[{"name":"Department of Legal Medicine Ehime University Graduate School of Medicine  Toon Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azumi","family":"Kuse","sequence":"additional","affiliation":[{"name":"Division of Legal Medicine Department of Environmental Health and Safety Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Kondo","sequence":"additional","affiliation":[{"name":"Division of Legal Medicine Department of Environmental Health and Safety Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motonori","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Division of Legal Medicine Department of Environmental Health and Safety Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mai","family":"Morichika","sequence":"additional","affiliation":[{"name":"Division of Legal Medicine Department of Environmental Health and Safety Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanako","family":"Nakagawa","sequence":"additional","affiliation":[{"name":"Division of Legal Medicine Department of Environmental Health and Safety Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Sakurada","sequence":"additional","affiliation":[{"name":"Division of Legal Medicine Department of Environmental Health and Safety Kobe University Graduate School of Medicine  Kobe Japan"},{"name":"Forensic Science Laboratory Hyogo Prefectural Police Headquarters  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Ueno","sequence":"additional","affiliation":[{"name":"Division of Legal Medicine Department of Environmental Health and Safety Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-27579-7_33"},{"key":"e_1_2_8_3_1","first-page":"821","volume-title":"Miller\u2019s anesthesia","author":"Vuyk J","year":"2015"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542\u2010197311000\u201000024"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0379\u20100738(88)90184\u20103"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS12804J"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/15.3.155"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac040065a"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/23.7.1306"},{"issue":"4","key":"e_1_2_8_10_1","first-page":"343","article-title":"Determination of optically active thiopental, thiamylal, and their metabolites in human urine","volume":"5","author":"Carroll FI","year":"1977","journal-title":"Drug Metab Dispos"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2116\/bunsekikagaku.35.3_245"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0378\u20104347(00)83026\u20101"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378\u20104347(94)00527\u2010C"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkx025"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-087780-8.00056-5"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2019.110136"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2019.03.048"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.legalmed.2012.04.008"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jaoac\/86.2.412"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aeq376"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010011\u20104656\u20105"},{"key":"e_1_2_8_22_1","first-page":"221","volume-title":"Analytical chemistry of PCBs","author":"Erickson MD","year":"1977"}],"container-title":["Journal of Forensic Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1556-4029.14965","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1556-4029.14965","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1556-4029.14965","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T13:03:00Z","timestamp":1692882180000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/1556-4029.14965"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,30]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1111\/1556-4029.14965"],"URL":"https:\/\/doi.org\/10.1111\/1556-4029.14965","archive":["Portico"],"relation":{},"ISSN":["0022-1198","1556-4029"],"issn-type":[{"value":"0022-1198","type":"print"},{"value":"1556-4029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,30]]},"assertion":[{"value":"2021-09-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}